سمیت متیل سولفونیل متان بر روی سلول های سرطانی معده (AGS)و کبد (HepG2)

عنوان انگليسی

Cytotoxicity of Methylsulfonylmethane (MSM) on Gastric Cancer (AGS) and Liver Cancer (HepG2) Cell Lines

خلاصه انگلیسی

Background: gastric cancer is the fourth most common cancer worldwide, It is a disease with a high death rate, making it the second most common cause of cancer death worldwide, It is more common in men and in developing countries. Liver cancer ranks fifth in frequency in the world, rates of liver cancer diagnosis are increasing all over the word. Gastric and liver cancers have been considered as a chemosensitive tumors for many years, no significant progress in their management has been resulted within the last two decades. Methylsulfonylmethane (MSM) also known as dimethyl sulfone is an organic form of sulfur with the formula of CH3SO2CH3. MSM has unique properties related to its chemical structure and biological activities.MSM is a hypoallergenic agent and can be dietary supplement to support proper joint, connective tissue and skin health. MSM supports collagen formation and maintain cell membrane flexibility. MSM is considered to be one of the least toxic substances in biology, similar in toxicity to water. The administration of MSM to human volunteers has been showed no toxic effects. In spite several experiments have shown that oral administration of MSM can protect rats against the onset of cancer. The researchers concluded that MSM significantly lengthens the time of tumor onset compared to the controls. Considering preventive effects of MSM on tumor onset and non toxic to healthy body and hence chemosensitive properties of gastric and liver tumors, we investigated cytotoxic effects of MSM on these two gastrointestinal cancers. Method and materials: MSM prepared in 50 mg/ml concentration with RPMI 1640. AGS and HepG2 cell lines provided from Pasteur Institute of Iran (Tehran, Iran) and plated in 96-well plates with the density of 1x104 per well in 200 μl of medium (RPMI 90% + FBS 10%) and incubated for an overnight (37C, 5%CO2, air humidified). plates were treated with MSM by initial concentration of 50 mg/ml with ¼ serial dilution and incubated for 72 hrs. Cytotoxicity of MSM was examined by MTT assay.. Results: MSM had shown cytotoxic effects on gastric and liver cancer cell lines at 72 hrs incubation time. Inhibitory concentration of 50% (IC50) of the MSM on AGS cell line was 12.15 mg/ml, and on HepG2 cell line was 28 mg/ml. Conclusion: MSM had cytotoxic effects on gastric and liver cancer cell lines. In this study MSM showed most cytotoxic effect against AGS cell line. Although MSM had cytotoxic effects on both gastric and liver cell lines but gastric cancer cell lines were more susceptible to MSM.

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