آیا عناصر توالی الحاقی (IS)در پیدایش سویه های بالینی سودوموناس آئروژینوزا مقاوم به کارباپنم در اردبیل نقش دارند؟

عنوان انگليسی

Whether Insertion Sequence (IS) elements are involved in the emergence of carbapenem-resistant Pseudomonas aeruginosa clinical strains in Ardabil?

خلاصه انگلیسی

Introduction: Carbapenems are the main antibiotics for the treatment of infections caused by multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa). The aims of this study were to determine the prevalence of gene encoding outer membrane porin protein (OprD) in carbapenem-resistant P. aeruginosa strains as well as to assess the role of insertion sequence (IS) elements in the inactivation of OprD porin and the emergence of carbapenem resistance. Methods: In the current study, imipenem-resistant (n=58), meropenem-resistant (n=42) and doripenem-resistant (n=23) P. aeruginosa strains were used. The presence of the oprD gene and IS elements were investigated using polymerase chain reaction (PCR) and sequencing methods. P. aeruginosa PAO1 strain was used as the positive control strain. Results: The prevalence of oprD gene among carbapenem-resistant P. aeruginosa strains was 96.5%. However, the prevalence of IS elements was 0%. Discussion & Conclusion: Our results showed that IS elements are not involved in the inactivation of outer membrane porin OprD and the emergence of carbapenem-resistant P. aeruginosa clinical strains in Ardabil city.

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