شناسایی مولکولی و تعیین مشخصات گونه های شیگلا حاوی ژنهای بتالاکتامازهای وسیع الطیف جدا شده از کودکان در شمال غرب ایران

صبور, سحر and تیمورپور, امیر and محمدشاهی, جعفر and پیری دوگاهه, هادی and تیمورپور, رقیه and عظیمی, طاهر and حسینعلی, زهرا (1401) شناسایی مولکولی و تعیین مشخصات گونه های شیگلا حاوی ژنهای بتالاکتامازهای وسیع الطیف جدا شده از کودکان در شمال غرب ایران. Molecular and Cellular Pediatrics ــ 9 (19). شاپا 2194-7791

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Molecular detection and characterization of Shigella spp. harboring extended‑spectrum β‑lactamase genes in children with diarrhea in northwest Iran

English Abstract

Shigellosis is one of the acute bowel infections and remains a serious public health problem in resource-poor countries. The present study aimed to survey the distribution of extended-spectrum β-lactamase (ESBL)-producing Shigella strains isolated from patients with diarrhea in northwest Iran. In the present cross-sectional study, from January 2019 to December 2020, 1280 fecal samples were collected from children with diarrhea in Ardabil, Iran. Multiplex PCR assay was applied for the presence of ipaH, invC, wbgZ, rfpB, and rfc genes to detect Shigella spp., Shigella sonnei, Shigella dysenteriae, Shigella flexneri, and Shigella boydii, respectively. Phenotypic detection of ESBL-producing isolates was carried out using the Double Disc Test (DDT). The frequency of main ESBL encoding genes including blaCTX-M, blaSHV, and blaTEM was detected using multiplex PCR. The genetic similarity of S. sonnei isolates was determined using ERIC PCR. A total of 49 Shigella isolates (3.8%; 49/1280) including 42 (85.7%) S. sonnei, 5 (10.2%) S. flexneri, and 2 (4%) S. dysenteriae were identified. S. boydii was not detected in any fecal samples. ESBLs were produced by 10.2% of Shigella spp. including 3 S. sonnei, 1 S. flexneri, and 1 S. dysenteriae. The ESBL encoding genes include blaCTX-M and blaTEM found in 65.3% and 61.2% of isolates, respectively. blaSHV gene was not detected in any isolates. The ERIC-PCR profiles allowed the differentiation of 42 S. sonnei strains into 6 clusters. Our study revealed a high frequency of ESBL-encoding genes among Shigella spp. in northwest Iran. The high prevalence of S. sonnei harboring ESBL genes, in the present work, is the main challenge for dysentery treatment, and this concern justifies the need for effective and regular monitoring of antibiotic usage among patients.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سحر صبور
نویسنده :امیر تیمورپور
نویسنده مسئول :جعفر محمدشاهی
نویسنده :هادی پیری دوگاهه
نویسنده :رقیه تیمورپور
نویسنده :طاهر عظیمی
نویسنده :زهرا حسینعلی
Additional Information:Indexed in: PubMed/PMC, Embase
Uncontrolled Keywords:گونه های شیگلا، شیگلا سونه ای ، بتالاکتامازهای وسیع الطیف، اسهال، ایران
کلیدواژه ها (انگلیسی):Shigella spp., Shigella sonnei, Extended-spectrum β-lactamase, Diarrhea, Iran
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:16480
Deposited By: Dr Roghayeh Teimourpour
Deposited On:23 Sep 1401 11:32
Last Modified:23 Dec 1401 10:51

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