بررسی سطح سرمی و پلی مورفیسم های ناحیه پروموتر ژن TGF-B در بیماران پره اکلامپسی و گروه کنترل

Title

Serum Level and polymorphisms of promoter region of transforming growth factor beta (TGF-β1) gene In Pre eclampsia and Control Group

English Abstract

Background & objectives: Pre eclampsia (PE) is pregnancy associated disorder with hypertension and proteinuria that cause neonatal and maternal morbidity and mortality. The current hypothesis regarding the etiology of PE is focused on deviation of immune responses and Type1/Type2 cytokines disequilibrium. Type2 cytokines appear to contribute to the maintenance of pregnancy by controlling the immune and endocrine systems. TGF-β1 plays important roles in immunregulation, immunedeviation, placental development, hypertension and renal function. presence of this cytokine in semen and pregnancy associated site(tissues) prompt us to evaluate association of this cytokine with PE aethiopathology.Methodes: In this investigation the polymorphisms of the TGF-β1 gene at promoter region positions -800 (G/A) and -509 (C/T) that affect expression of this cytokine were studied in 142 PE and 140 normal pregnant female subjects by PCR-RFLP.Also TGF-β1 serum levels were determined by ELISA method. Results: It was shown that at position -800 (G/A) polymorphism, genotype distribution and allele frequencies between PE patients(GG 73.9%; GA 21.1%; AA 4.93%) and normal control(GG 70%;GA 28.6%;AA 1.4%) showed no significant differences. but AA genotype of -800 (G/A) position were higher in PE patients than control group. In the case of the -509 (C/T) polymorphism, 28.2% of patients, 25% of normal controls, were homozygote CC. While 41.5% of cases and 44.3% of normal controls, were heterozygote CT, 30.3% of PE and 30.7% of normal controls, were homozygote TT respectively. Statistical analysis showed no significan differences of allele and genotype distributions frequencies between PE cases and normal controls at position-509 (C/T). but CC genotype at this posaition were higher in PE patients than control group. Mean TGF-β1 serum levels were 62.14 and 45.7 ng/ml in PE patients and control group respictively. Conclusion: the promoter region polymorphisms of TGF-β1 may not be associated with PE, but serum levels of this cytokine may contribute in the etiopathology of PE by different mechanisms. Future studies need to clarify the association of TGF-β1with PE.

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