فراوانی اینتگرون و ژنهای ESBL در سویه های اشرشیا کلی مقاوم به چند دارو جدا شده از عفونت دستگاه ادراری ، اردبیل، ایران

برزگر, سهیلا and ارزنلو, محسن and تیمورپور, امیر and اسماعیلی زاد, مجید and یوسفی پور, مهدی and محمدشاهی, جعفر and تیمورپور, رقیه (1400) فراوانی اینتگرون و ژنهای ESBL در سویه های اشرشیا کلی مقاوم به چند دارو جدا شده از عفونت دستگاه ادراری ، اردبیل، ایران. Iranian Journal of Medical Microbiology ــ 16 (1). pp. 56-65. شاپا 2345-4342

Text - Published Version

Official URL: https://ijmm.ir/article-1-1472-en.html


Prevalence of the Integrons and ESBL Genes in Multidrug-Resistant Strains of Escherichia coli Isolated from Urinary Tract Infections, Ardabil, Iran

English Abstract

Background and Aim: Integrons play an essential role in disseminating drug resistance genes among bacteria. The aim of this study was to determine the prevalence of integrons, and Extended-Spectrum β-Lactamase (ESBL) genes in Escherichia coli (E. coli) isolates collected from patients with urinary tract infection (UTI) referred to teaching hospitals in Ardabil, Iran. Materials and Methods: In this descriptive, cross-sectional study (2017-2018), 163 isolates of E.coli were collected from patients with UTI. The drug susceptibility pattern of these isolates to 12 common antibiotics was investigated using the disk diffusion method based on CLSI guidelines. The prevalence of class 1, 2 ,3 integrons and ESBL genes was verified by the PCR method. Finally, the genetic variation of isolates was analyzed using the ERIC-PCR method. Results: Of 163 isolates, 138 (84.7%) isolates were multidrug-resistance (MDR) strains. The lowest and highest antibiotic resistance was reported to nitrofurantoin and ampicillin, with a resistance rate of 1.2% and 89.6%, respectively. The incidence of class 1 and 2 integrons was obtained in 39.9% and 14.1% of the isolates, respectively. Class 3 integron was not found in any of the isolates. Based on the ERIC-PCR fingerprinting method, 4 ERIC types were detected. Conclusion: Our study showed that E. coli isolates taken from patients mainly were MDR strain and resistant to many of the common antibiotics used to treat urinary tract infections. Using the correct dose of medication and multidrug therapy would be effective in reducing the incidence of antibiotic resistance.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سهیلا برزگر
نویسنده :محسن ارزنلو
نویسنده :امیر تیمورپور
نویسنده :مجید اسماعیلی زاد
نویسنده :مهدی یوسفی پور
نویسنده :جعفر محمدشاهی
نویسنده مسئول :رقیه تیمورپور
Additional Information:Indexed in: Scopus, DOAJ
Uncontrolled Keywords:اشرشیاکلی، اینتگرون، مقاومت چند دارویی، عفونت ادراری
کلیدواژه ها (انگلیسی):Escherichia coli, Integron, Multi-drug resistance, UTI
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:16784
Deposited By: Dr Roghayeh Teimourpour
Deposited On:10 Dec 1401 10:18
Last Modified:23 Dec 1401 10:52

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