کرمی, پری ، باقری, حسن ، جوهری اهر, محمد ، خوش سفر, حسین ، Arduini, Fabiana ، افخمی, عباس (1398) حسگر ایمنی مقاومت سنجی برای تشخیص همزمان زودهنگام نشانگرهای آنتی ژن اختصاصی پروستات و میوگلوبین براساس پلیمر چاپ مولکولی. TALANTA ــ 202 (2019). ص.ص.111-122. شاپا 0039-9140
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آدرس اینترنتی رسمی : https://www.sciencedirect.com/science/article/abs/...
عنوان انگليسی
Dual-modality impedimetric immunosensor for early detection of prostate-specific antigen and myoglobin markers based on antibody-molecularly imprinted polymer
خلاصه انگلیسی
A new dual-modality immunosensor based on molecularly imprinted polymer (MIP) and a nanostructured biosensing layer has fabricated for the simultaneous detection of two important markers including prostate-specific antigen (PSA) and myoglobin (Myo) in human serum and urine samples. In the first step, 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP) was self-assembled on a gold screen printed electrode (SPE). Then, the target proteins were attached covalently to the DSP-SPE. The imprinted cocktail polymer ((MIP(PSA, Myo)-SPE)) was synthesized at the SPE surface using acrylamide as monomer, N,N'-methylenebisacrylamide as a crosslinker, and PSA and Myo as the templates, respectively. The MIP-SPE was specific for the impedimetric sensing of PSA and Myo. After that, a nanocomposite (NCP) was synthesized based on the decorated magnetite nanoparticles with multi-walled carbon nanotube, graphene oxide and specific antibody for PSA (Ab). Then, NCP incubated with (MIP(PSA, Myo)-SPE. The modified electrodes and synthesized nanoparticles were characterized using electrochemical impedance spectroscopy, dynamic light scattering, surface plasmon resonance and scanning electron microscopy. The limits of detections were found to be 5.4 pg mL-1 and 0.83 ng mL-1 with the linear dynamic ranges of 0.01-100 and 1-20000 ng mL-1 for PSA and Myo, respectively. The ability of proposed biosensor to detect PSA and Myo simultaneously with high sensitivity and specificity offers a powerful opportunity for the new generation of biosensors. This dual-analyte specific receptors-based device is highly desired for the integration with lab-on-chip kits to measure a wide panel of biomarkers present at ultralow levels during early stages of diseases progress.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | پری کرمی |
نویسنده مسئول : | حسن باقری |
نویسنده : | محمد جوهری اهر |
نویسنده : | حسین خوش سفر |
نویسنده : | Fabiana Arduini |
نویسنده : | عباس افخمی |
ضریب تاثیر و نمایه مجلات: | IF: 6.057 Indexed in: ISI, PubMed/Medline, Scopus |
کلیدواژه ها (فارسی): | حسگر ایمنی غیرمجاز؛ پلیمر مولکولی پرینت شده؛ نشانگر میوگلوبین؛ آنتی ژن اختصاصی پروستات؛ تعیین همزمان |
کلیدواژه ها (انگلیسی): | Impedimetric immunosensor; Molecularly imprinted polymer; Myoglobin marker; Prostate-specific antigen; Simultaneous determination |
موضوعات : | QV فارماکولوژی |
کد شناسایی : | 17174 |
ارائه شده توسط : | دکتر محمد جوهری اهر |
ارائه شده در تاریخ : | 03 تیر 1402 09:37 |
آخرین تغییر : | 03 تیر 1402 09:37 |
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