فیض پور, سیما ، حسینی یزدی, سید ابوالفضل ، صفرزاده, الهام ، برادران, بهزاد ، Dusek, Michal ، Poupon, Morgane (1402) یک لیگاند پایه تیوسمی کاربازون شیف محلول در آب جدید و کمپلکس های آن به عنوان عوامل ضد سرطان بالقوه و تصویربرداری فلورسانس سلولی. JBIC Journal of Biological Inorganic Chemistry ــ 28 (5). ص.ص.457-472. شاپا 0949-8257
متن کامل
![]() |
متنی
- نسخه چاپ شده
محدود به فقط پرسنل سامانه 4MB |
آدرس اینترنتی رسمی : https://link.springer.com/article/10.1007/s00775-0...
عنوان انگليسی
A novel water-soluble thiosemicarbazone Schiff base ligand and its complexes as potential anticancer agents and cellular fluorescence imaging
خلاصه انگلیسی
A novel fluorescent ligand (H2LCl⋅1.5CH3OH, 1) was synthesized and metal complexes of 1 with Mn(II), Fe(III), Ni(II), Cu(II), and Zn(II) were obtained as Mn(HL)2Cl2 (2), Fe(HL)2Cl3⋅3H2O (3), Ni(L)(HL)Cl⋅8H2O (4), Cu(HL)Cl2⋅4H2O (5), Zn(H2L)Cl3 (6), respectively. These compounds were identified by spectroscopic methods, elemental analysis, molar conductivity, and single-crystal X-ray crystallography. According to the crystal structure of 4 nickel (II), center is surrounded by two ligands in a distorted octahedral geometry. The ligand and its complexes are soluble in water and have excellent stability. In vitro anti-proliferative activity of these compounds was evaluated against human breast adenocarcinoma (MCF-7) and human lipo-sarcoma (SW-872) as cancer cells and human fibroblasts (HFF-2) as normal cells by MTT assay. Interestingly, complex 5 exhibited excellent activity against both cancer cells with low IC50 value 22.18 ± 0.35 μg/mL (35.66 ± 0.56 μM) for SW-872 and 79.41 ± 3.54 μg/mL (127.6 ± 5.69 μM) for MCF-7 among the compounds and in comparison with paclitaxel (PTX) which acts finely. Morphological changes were evaluated by flow cytometry that revealed apoptosis is the main cause of cell death. Likewise, cell cycle studies indicated the cell cycle arrest in the G1 and S phases for complex 5 against MCF-7 and SW-872 cancer cells, while complex 6 could arrest the MCF-7 and SW-872 cells in G2 and G1 phases, respectively. All of the compounds are fluorescent which enabled us to monitor the uptake and intracellular distribution in living human cancer cells by fluorescence microscopy.
نوع سند : | مقاله |
---|---|
زبان سند : | انگلیسی |
نویسنده اول : | سیما فیض پور |
نویسنده مسئول : | سید ابوالفضل حسینی یزدی |
نویسنده : | الهام صفرزاده |
نویسنده : | بهزاد برادران |
نویسنده : | Michal Dusek |
نویسنده : | Morgane Poupon |
ضریب تاثیر و نمایه مجلات: | IF: 3 Indexed in: ISI, PubMed/Medline, Scopus |
کلیدواژه ها (انگلیسی): | Anticancer activity; Apoptosis; Cell cycle; Cytotoxicity; Flow cytometry; X-ray crystallography. |
موضوعات : | QW میکروب شناسی و ایمنی شناسی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش ايمنولوژی |
کد شناسایی : | 17271 |
ارائه شده توسط : | دکتر الهام صفرزاده |
ارائه شده در تاریخ : | 30 آبان 1402 08:07 |
آخرین تغییر : | 30 آبان 1402 08:07 |
فقط پرسنل کتابخانه صفحه کنترل اسناد