MicroRNA-320 اثر جایگزینی بر رشد و مهار تکثیر در رده سلولی سرطان معده (AGS)

عنوان انگليسی

MicroRNA-320 a replacement effect on growth and proliferation inhibition in the gastric cancer cell line (AGS)

خلاصه انگلیسی

Background: Gastric cancer is a major cause of cancer mortality worldwide. In this study, we transfected the miR-320a mimic into gastric cancer cells by the Electroporation technique and investigated the effects of this intervention on the growth and proliferation inhibition of gastric cancer cells. Method and materials: The gastric cancer AGS cell line was cultured in an incubator at(37C with moisture 95% and 5% Co2) using RPMI-1640 culture medium with 10% FBS. After cell preparation, cell count was performed, then cells were transferred to covet for transfection. The Electroporation technique was used to transfect the miR-320a mimic and negative control (NC) into the cells. To evaluate the change in the expression level of miR-320a,qRT-PCR was performed. Cell viability was investigated using the MTT assay. The miR-320a mimic transfected, negative control, and un-transfected cells were seeded in 96 well plates and incubated at 37C for 24 hours. After that, MTT was added to wells. Following 4 hours of incubation, DMSO was inserted into the samples to dissolve the formazan crystal. Eventually, the spectrophotometric plate reader was used for UV absorbance measurement at 570 nm. Results: Based on the viability of cells, the optimum electroporation voltage for the AGS cell line was 230v. According to the MTT test results, the survival rate of AGS cells decreases with increasing the dose of miR-320a mimic, which indicates that the effect of miR-320a mimic on the AGS cell line is dose-dependent. IC50 of microRNA 320a for the AGS cell line was obtained 60pmol in 24 hours. These findings indicate that transfected miR-320a mimic could suppress AGS cells' proliferation compared to NC and the control group. The obtained results inqRT‐PCR indicated that the expression level of miR- 320a levels in transfected AGS cells was increased compared to the NC and control groups. Conclusion: As expected, these results confirm the tumor-suppressive effect of miR-320a in the AGS gastric cancer cell line by reducing cell survival and proliferation. Understanding this will allow us to take a big step forward in the treatment of gastric cancer. Keyword: Gastric cancer; microRNA-320a; Apoptosis; Metastasis

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