تعدیل اتوفاژی در سلول های سرطانی معده و حساسیت به 5-فلوراوراسیل با درمان ترکیبی با نانوذرات Se-FA

عنوان انگليسی

Modulation of Autophagy in Gastric Cancer Cells and Sensitization to 5-Fluorouracil by Combination Therapy with Se–FA Nanoparticles

خلاصه انگلیسی

Recently, nanoparticle (NP)-based drugs have been developed to increase the efficiency of existing cancer treatment approaches; however, how NPs interact with cellular systems is still uncertain. In this study, we have examined the ability of Se–FA NPs to enhance the chemotherapeutic effect of 5-fluorouracil (5-FU) in human AGS gastric cancer cells as well as their ability alone and in combination with 5-FU to modulate autophagy. The prepared NPs were characterized using Fourier-transform infrared spectroscopy, dynamic light scattering, and scanning electron microscope. Cytotoxicity and autophagy-modulating activity of NPs were determined using MTT, q-PCR, and western blot methods. The size and surface charge of NPs were determined to be ~ 90.4 nm and − 53.8 mV, respectively. Se–FA NPs enhance the cytotoxicity of 5-FU (up to 4.1-fold) and consequently inhibit autophagy flux in AGS cells. These findings confirm the therapeutic potential of combining Se–FA NPs with 5-FU in an in vitro gastric cancer model and may suggest an approach to overcome chemotherapy resistance in cancer.

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