بررسی فاکتورهای ویرولانس هلیکوباکتر پیلوری جدا شده از معده بیوپسی

عنوان انگليسی

Exploring virulence factors of Helicobacter pylori isolated from gastric biopsy

خلاصه انگلیسی

Background Helicobacter pylori (H. pylori) colonizes human gastric mucosa and is classifed as class one carcinogenic bacteria. In this regard, this study aimed to detect major virulence factors in H. pylori strains recovered from gastric biopsy in patients referred to Aras Clinique in Ardabil, northwest of Iran (2019–2021). Materials and methods In this descriptive-cross sectional study, 287 dyspeptic patients were included. For bacterial isolation, gastric biopsy specimens (n=287) were taken from gastric antrum, then aseptically were cultured on the selective medium and incubated at 37C in microaerophilic conditions for 3-5 days. Results 25.18% of all (n=70) patients were found to be infected with H. pylori upon endoscopy. Of them, 9 patients (12.857%) and 2 patients (2.875%) had peptic ulcer disease and gastric cancer respectively. According to the diferent patterns of virulence factors, 57 virutypes were identifed in which oipA-vacAs1-vacAm2 (3, 4.28% n=) and oipA-vacAs1- vacAs2-vacAm2 (3, 4.28% n=) were the most common patterns. The simultaneous presence of vacAS2, vacAm2 and hopQ2 genes was observed in both patients with gastric cancer. OipA (n=562.5%), VacAs1 (n=6.75%), VacAs2 (n=6.75%), and VacAm2 (n=787.5%) were found to be the most prevalent virulence factor. Conclusion According previous studies, it is confrmed that the cagPAI gene cluster and vacA gene alleles are strongly correlated with gastritis and gastrointestinal tract adenocarcinomas. Our study indicated that 50% of the indigenous strains of H. pylori harbor these oncogenic genes and they are hypervirulent.

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