مقایسه روش الیزا و آزمون استاندارد لوله ای (رایت) جهت تشخیص بروسلوز انسانی

عنوان انگليسی

Comparison of ELISA and STA Tests for Diagnosis of Human Brucellosis

خلاصه انگلیسی

Introduction: Brucellosis is endemic in Iran Since it affects many organs and the symptoms are non-specific, the diagnosis by clinical findings is difficult and may be easily missed. Many serological tests have been used for the diagnosis of human brucellosis. This study determined the diagnostic value of the Brucella standard tube agglutination test (SAT), with that of ELISA (Brucella specific IgG and IgM) tests in suspected patients with brucellosis. Materials and Methods: Ten patients with brucellosis who had positive blood cultures for Brucella species, and 47 suspected patients were included in the study. Serum samples collected from these patients were tested by SAT and ELISA (Brucella specific IgG and IgM) tests. Results: In the patients with blood culture positive, 4 of 10 samples gave positive results with SAT, 7and 8 with ELISA IgM and IgG, and all patients were positive with ELISA (IgG + IgM). Out of 47 with blood culture negative, ELISA (IgG + IgM) and SAT were positive in 28 and 18, patients respectively. Conclusion: According to this study, none of serological tests alone was positive in all suspected and confirmed patients with brucellosis. For this reason, we strongly suggested the use of combination of tests for diagnosis of brucellosis. The sensitivity of ELISA (IgG + IgM) was higher than SAT test .

Document Downloads