مطالعه سمیت متیل سولفونیل متان بر روی رده های سلولی سرطانی دستگاه گوارش (AGS, HepG2 و KEYSE-30)

جعفری, ناصر and بهلولی, شهاب and محمدی, پرهام سعداله and مأذنی, محمد (1391) مطالعه سمیت متیل سولفونیل متان بر روی رده های سلولی سرطانی دستگاه گوارش (AGS, HepG2 و KEYSE-30). Journal of gastrointestinal cancer ــ 43 (3). pp. 420-425. شاپا 1941-6636

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Official URL: http://www.springerlink.com/content/d2tn8473x84qj9...


Cytotoxicity of Methylsulfonylmethane on Gastrointestinal (AGS, HepG2, and KEYSE-30) Cancer Cell Lines

English Abstract

PURPOSE: This study was conducted to assay cytotoxic effects of methylsulfonylmethane (MSM) on gastrointestinal cancer cell lines. METHODS: Human gastric carcinoma (AGS), human hepatocellular carcinoma (HepG2), and human esophageal squamous cell carcinoma (KYSE-30) cancer cell lines were treated by MSM and incubated for 24, 48, and 72 h. Cytotoxicity was examined through MTT, neutral red uptake, and protein measurement assays. Ethidium bromide/acridine orange (EB/AO) staining was used for apoptotic cell detection. A diamidino-2-phenylindole staining method was used to analysis cell cycle by flow cytometry. RESULTS: IC(50) of MSM on AGS, HepG2, and KYSE-30 cell lines were 28.04, 21.87 and 27.98 mg/ml after 72 h, respectively. The EB/AO staining showed an increase in apoptotic cells. Cell cycle analysis showed a significant increase in cell density at G2/M phase. CONCLUSION: MSM had cytotoxic effect on cancer cell lines but HepG2 cell line was more susceptible. This study suggests that MSM may induce cytotoxic effect on gastrointestinal cancer cell lines by apoptosis and cell cycle arrest.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :ناصر جعفری
نویسنده مسئول :شهاب بهلولی
Additional Information:Abstracted/Indexed in:1-PubMed/Medline, 2-SCOPUS,Chemical Abstracts Service (CAS), EMBASE, 3-Academic OneFile, AGRICOLA,Expanded Academic, Gale, Google Scholar, Health Reference Center Academic, IBIDS, OCLC,Summon by Serial Solutions ISSN: 1941-6628 (print version)
Uncontrolled Keywords:متیل سولفونیل متان ، MSM ، رده سلول سرطانی ، سمیت سلولی ، آپپتوز
کلیدواژه ها (انگلیسی):Methylsulfonylmethane . MSM . Cancer cell lines . Cytotoxicity . Apoptosis
Subjects:QV pharmacology
WI Digestive System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
Faculty of Medicine > Department of Basic Sciences > Department of Pharmacology
ID Code:2444
Deposited By: MS Soghra Golmaghani
Deposited On:28 Mar 1390 12:50
Last Modified:12 Dec 1397 13:39

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