القای نورونی و هویت مکانی سلولهای بنیادی جنینی انسانی به دنبال هم کشتی با نوتوکورد و سومایت جنین جوجه

صالحی, حسین and کربلایی, خدیجه and رضوي, شهناز and تنهايي, سميه and نعمت الهی, مرضیه and سقا, محسن and نصر اصفهاني, محمد حسين and بهاروند, حسين (1390) القای نورونی و هویت مکانی سلولهای بنیادی جنینی انسانی به دنبال هم کشتی با نوتوکورد و سومایت جنین جوجه. International Journal of Developmental Biology ــ 55 (3). pp. 321-326. شاپا 1696-3547

Text - Published Version

Official URL: http://www.ijdb.ehu.es/web/paper.php?doi=103185hs


Neuronal induction and regional identity by co-culture of adherent human embryonic stem cells with chicken notochords and somites

English Abstract

The role of somites and notochords in neuroectoderm differentiation from the embryonic ectoderm and its subsequent patterning into regional compartments along rostro-caudal and dorso-ventral axes, especially in humans, remains elusive. Here, we demonstrate the co-culture effect of somites and notochords isolated from chicken embryos on the neuronal differentiation and regional identity of an adherent culture of human embryonic stem cells (hESCs). Notochord increased the efficiency and speed of neuronal induction, whereas somites had a weak neuronal inducing effect on hESCs. However, a synergistic effect was not observed when notochords and somites were used together. Moreover, in somite and notochord co-culture groups, hESCs-derived neuronal cells expressed HOXB4, OTX2, IRX3 and PAX6, indicative of dorsal hindbrain and ventral anterior identities, respectively. Our results reveal the influence of embryonic notochord and somite co-culture in providing neuronal induction as well as rostro-caudal and dorso-ventral regional identity of hESCs-derived neuronal cells. This study provides a model through which in vivo neuronal induction events may be imitated

Item Type:Article
زبان سند : انگلیسی
نویسنده :محسن سقا
نویسنده مسئول :محمد حسين نصر اصفهاني
نویسنده مسئول :حسين بهاروند
Additional Information:5years: Impact factor:3.253 Indexing : 1-Science Citation Index, Science Citation Index Expanded 2- Current Contents , BIOSIS Previews 3-Life Sciences , Zoological Record
کلیدواژه ها (انگلیسی):chicken , co-culture , hESC , Neuronal induction , Notochord , Somite
Subjects:QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:2466
Deposited By: Dr Mohsen Sagha
Deposited On:18 Apr 1390 07:57
Last Modified:01 Nov 1391 12:50

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