تخلیص و جداسازی سلولهای عصبی نابالغ از رده های سلولهای بنیادی عصبی

آذری, حسن and اوزبورن, جفری and یاسودا, تاکاهیرو and گل محمدی, محمد قاسم and رحمان, مریم and دلی رول, لوئیک and اسفندياري, ابراهيم and آدامز, دیوید and شفلر, جورن and استیندلر, دنیس and رینولدز, برنت (1390) تخلیص و جداسازی سلولهای عصبی نابالغ از رده های سلولهای بنیادی عصبی. PloS ONE ــ 6 (6). e20941. شاپا 1932-6203

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Official URL: http://www.plosone.org/article/info%3Adoi%2F10.137...


Purification of Immature Neuronal Cells from Neural Stem Cell Progeny

English Abstract

Large-scale proliferation and multi-lineage differentiation capabilities make neural stem cells (NSCs) a promising renewable source of cells for therapeutic applications. However, the practical application for neuronal cell replacement is limited by heterogeneity of NSC progeny, relatively low yield of neurons, predominance of astrocytes, poor survival of donor cells following transplantation and the potential for uncontrolled proliferation of precursor cells. To address these impediments, we have developed a method for the generation of highly enriched immature neurons from murine NSC progeny. Adaptation of the standard differentiation procedure in concert with flow cytometry selection, using scattered light and positive fluorescent light selection based on cell surface antibody binding, provided a near pure (97%) immature neuron population. Using the purified neurons, we screened a panel of growth factors and found that bone morphogenetic protein-4 (BMP-4) demonstrated a strong survival effect on the cells in vitro, and enhanced their functional maturity. This effect was maintained following transplantation into the adult mouse striatum where we observed a 2-fold increase in the survival of the implanted cells and a 3-fold increase in NeuN expression. Additionally, based on the neural-colony forming cell assay (N-CFCA), we noted a 64 fold reduction of the bona fide NSC frequency in neuronal cell population and that implanted donor cells showed no signs of excessive or uncontrolled proliferation. The ability to provide defined neural cell populations from renewable sources such as NSC may find application for cell replacement therapies in the central nervous system.

Item Type:Article
زبان سند : انگلیسی
نویسنده :حسن آذری
نویسنده :جفری اوزبورن
نویسنده :تاکاهیرو یاسودا
نویسنده :محمد قاسم گل محمدی
نویسنده :مریم رحمان
نویسنده :لوئیک دلی رول
نویسنده :ابراهيم اسفندياري
نویسنده :دیوید آدامز
نویسنده :جورن شفلر
نویسنده :دنیس استیندلر
نویسنده مسئول :برنت رینولدز
Additional Information:Impact factor:4.411 Indexing and Archiving: 1- PubMed, MEDLINE, Web of Science, 2- Scopus, Chemical Abstracts Service (CAS), EMBASE, 3-Google Scholar, the AGRICOLA, PsycINFO, Zoological Records, FSTA (Food Science and Technology Abstracts), GeoRef, PubMed Central
کلیدواژه ها (انگلیسی):neural stem cell ; immature neuron ; flow cytometry ; purification ; BMP-4 ; 29 transplantation 30
Subjects:QS Human Anatomy
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:2472
Deposited By: Dr Mohammad Gasem Golmohammani
Deposited On:21 Apr 1390 11:40
Last Modified:03 Nov 1391 19:49

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