تاثیر دی متیل سولفون بر سلولهای سرطان دستگاه گوارش (AGS, HepG2 and KYSE-30) و ارزیابی چرخه سلولی توسط فلوسایتومتری

Title

The Effect of Dimethyl Sulfone on Gastrointestinal Cancer Cell Lines (AGS, HepG2 and KYSE-30) and Flow Cytometric Analysis of the Cell Cycle

English Abstract

Background: Methylsulfonylmethane (MSM) is a dietary supplement to support healthy body. It is considered to be one of the least toxic substances in biology. Considering preventive effects of this substance on tumor onset and its low toxicity to healthy body we investigated in vitro cytotoxic effects of methylsulfonylmethane on cancer cell lines. Methods: AGS, HepG2 and KYSE-30 cancer cell lines were treated by MSM by initial concentration of 50 mg/ml with ¼ serial dilutions and incubated for 24, 48 and 72 hours. Cytotoxicity was examined through MTT, neutral red uptake and protein measurement assays. EB/AO staining was used for apoptotic cell detection. A DAPI staining method was used to analysis cell cycle by flow cytometry. Results: IC50 of the MSM on AGS, HepG2 and KYSE-30 cell lines were 28.04, 21.87 and 27.98 mg/ml after 72 hours respectively. The EB/AO staining showed an increase in apoptotic cells after MSM treatment. The flow cytometry cell cycle analysis showed a significant increase in cell density at G2/M phase. Conclusion: MSM had cytotoxic effect on cancer cell lines but HepG2 cell line was more susceptible. This study provides the evidence that MSM may induce cytotoxic effect on cancer cell lines by apoptosis and it could be of value in realm of chemotherapy.

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