ارتباط ميان فعاليت تلومراز با بيان زير واحد RNA آن در بيماران مبتلا به سرطان پستان

حسینی اصل, سید سعید and مدرسی, محمد حسین and عطری, مرتضی and سلحاب, محمد and مکبل, کفاه and مهدی پور, پروین (1384) ارتباط ميان فعاليت تلومراز با بيان زير واحد RNA آن در بيماران مبتلا به سرطان پستان. Journal of carcinogenesis ــ 5 (17). pp. 1-6. شاپا 1477-3163

Text - Published Version

Official URL: http://www.carcinogenesis.com/article.asp?issn=147...


The association between telomerase activity and expression of its RNA component (hTR) in breast cancer patients: the importance of DNase treatment

English Abstract

Telomerase is a ribonucleoprotein enzyme that compensates for the telomere length shortening which occurs during the cell cycle. Telomerase activity has been detected in most tumours but not in somatic cells. However, hTR; the RNA component of telomerase; has been reported to be universally expressed in both cancerous and non-cancerous tissues. Tumour samples from 50 patients with primary invasive breast cancer were collected. The TRAP assay was used to detect telomerase activity. RT-PCR on cDNA and DNased cDNA samples and control groups was used to detect the expression of hTR, GAPDH and PGM1 genes. Seventy-two percent of samples showed telomerase activity. DNA contamination was detected in 36 (72%) of RNA samples. Without performing DNase treatment, 49 (98%) of all samples showed hTR expression, but with the application of this strategy, hTR expression decreased from 98% to 64%. A significant association (p < 0.001) between hTR expression and telomerase activity was observed. Among the 32 hTR positive samples, 30 had telomerase activity and among the 18 hTR negative samples, telomerase activity was observed in 6 cases. Thus the application of this strategy could provide an applicable tool to use instead of the TRAP assay thus facilitating telomerase research in cancer genetic investigations.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سید سعید حسینی اصل
نویسنده :محمد حسین مدرسی
نویسنده :مرتضی عطری
نویسنده :محمد سلحاب
نویسنده :کفاه مکبل
نویسنده مسئول :پروین مهدی پور
Additional Information: indexed: CAB Abstracts, Caspur, CNKI (China National Knowledge Infrastructure), DOAJ, EBSCO Publishing's Electronic Databases, Excerpta Medica / EMBASE, Expanded Academic ASAP, Genamics JournalSeek, Global Health, Google Scholar, Health & Wellness Research Center, Health Reference Center Academic, Hinari, Index Copernicus, National Science Library, OpenJGate, PrimoCentral, ProQuest, Pubmed Central, Scimago Journal Ranking, SCOLOAR, SCOPUS, SIIC databases, Summon by Serial Solutions, Tropical Diseases Bulletin, Ulrich's International Periodical Directory
Uncontrolled Keywords:سرطان پستان، hTR، فعاليت تلومرازي ، مواجهه با DNase
کلیدواژه ها (انگلیسی):Breast cancer, hTR, Telomerase activity, DNase treatment
Subjects:QY Clinical Pathology
WB Practice of Medicine
WP Gynecology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Genetics
ID Code:2541
Deposited By: Dr S Saied Hosseini-Asl
Deposited On:27 Mar 1392 08:18
Last Modified:10 Jul 1393 08:49

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