تغییر باقیمانده های اسیدی موجب احیا فعالیت چاپرونی بتا کازئین در اندرکنش با لیزوزیم میشود

موسوی موحدی, علی اکبر and رجب زاد, حلیمه and امانی, مجتبی and نوروزیان, داریوش and زارع, کریم and هادی علیجانوند, حمید and شریفی زاده, احمد and پورساسان, نغمه and احمد, فیضان and شیبانی, نادر (1390) تغییر باقیمانده های اسیدی موجب احیا فعالیت چاپرونی بتا کازئین در اندرکنش با لیزوزیم میشود. International journal of biological macromolecules ــ 49 (4). pp. 616-621. شاپا 0141-8130

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Official URL: http://www.sciencedirect.com/science/journal/01418...


Acidic residue modifications restore chaperone activity of β-casein interacting with lysozyme.

English Abstract

An important factor in medicine and related industries is the use of chaperones to reduce protein aggregation. Here we show that chaperone ability is induced in β-casein by modification of its acidic residues using Woodward's Reagent K (WRK). Lysozyme at pH 7.2 was used as a target protein to study β-casein chaperone activities. The mechanism for chaperone activity of the modified β-casein was determined using UV-vis absorbencies, fluorescence spectroscopy, differential scanning calorimetry and theoretical calculations. Our results indicated that the β-casein destabilizes the lysozyme and increases its aggregation rate. However, WRK-ring sulfonate anion modifications enhanced the hydrophobicity of β-casein resulting in its altered net negative charge upon interactions with lysozyme. The reversible stability of lysozyme increased in the presence of WRK-modified β-casein, and hence its aggregation rate decreased. These results demonstrate the enhanced chaperone activity of modified β-casein and its protective effects on lysozyme refolding.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :علی اکبر موسوی موحدی
Additional Information:Impact Factor: 2.502 Abstracting and Indexing : 1- MEDLINE,Science Citation Index Expanded,Science Citation Index 2-Scopus ,Current Contents, EMBASE,BIOSIS Previews 3- EMBiology, Elsevier BIOBASE , FSTA ,Polymer Contents Life Sciences
کلیدواژه ها (انگلیسی)::Modified β-casein; Lysozyme; Hydrophobicity; Woodward's Reagent K; Electrostatic potential; Accessible surface area
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:2624
Deposited By: Dr Mojtaba Amani
Deposited On:27 Jun 1390 09:16
Last Modified:20 Jan 1393 12:41

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