title

القای مرگ سلولی برنامه ریزی شده در سلولهای سرطان دستگاه گوارش توسط متیل سولفونیل متان

جعفری, ناصر and بهلولی, شهاب (1390) القای مرگ سلولی برنامه ریزی شده در سلولهای سرطان دستگاه گوارش توسط متیل سولفونیل متان. در: دوازدهمين کنگره بيوشيمی ايران و چهارمين کنگره بين المللی بيوشيمی و بيولوژی مولکولی, 15 تا 18 شهريور 1390, ایران - مشهد.

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Title

poptosis induction of methysulfonylmethane in humangastrointestinal cancer cell lines

English Abstract

Introduction: Methylsulfonylmethane (MSM) is an organic sulfur containing compound that occurs naturally in a ariety of fruits, vegetables and unpasteurized milk. It has been used as a supplement in the last two decades. Recently it has been suggested that MSM has clinical potential as a non-toxic agent effective against metastatic melanoma. Considering the preventive effects of this substance on tumor onset and nontoxic to healthy body we have investigated in vitro effects of methylsulfonylmethane on apoptosis induction in human gastrointestinal cancer cell lines. Methods: The human cancer cell lines including, AGS, HepG2 and KYSE30 were cultured and incubated until confluence. The cells were removed and seeded in 96-well plates at a density of 1×103 cells/well and incubated overnight, and then treatedwith (21–30) g/mlMSM. To stain apoptotic cells, the plateswere centrifuged. The EB/AOdye mixwas prepared in PBS and 10 μlwas added to eachwell and cellswere viewed and counted under an inverted fluorescence microscope. Results: The results showed that after incubation at (21–29) mg/ml of MSM for 24 h the cells displayed a series of morphological changes including condensation and fragmentation of chromatin and nucleus, and formation of apoptotic bodies which were designated as typical evidence of apoptotic bodies. In contrast control cells exhibited a normal appearance. There was a significant increase of apoptotic cells in cancer cells after treating with MSM. Conclusion: The results demonstrated that MSM exerts its anticancer and cytotoxic effect by inducing apoptotic cell death.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده مسئول :ناصر جعفری
نویسنده :شهاب بهلولی
Additional Information:doi:10.1016/j.clinbiochem.2011.08.1066
کلیدواژه ها (انگلیسی):Apoptosis , Methylsulfonylmethane , Cancer
Subjects:QU Biochemistry
QV pharmacology
WI Digestive System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
Faculty of Medicine > Department of Basic Sciences > Department of Pharmacology
ID Code:2659
Deposited By: Mr Naser Jafari
Deposited On:04 Jul 1390 11:25
Last Modified:28 Nov 1391 09:39

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