روش نوین برای کشت سلول شوآن موش صحرایی بالغ با درجه خلوص بالا

نیاپور, علی and کرمعلی, فرشته and کربلایی, خدیجه and کیانی, عباس and مردانی, محمد and نصر اصفهاني, محمد حسين and بهاروند, حسين (1389) روش نوین برای کشت سلول شوآن موش صحرایی بالغ با درجه خلوص بالا. Biotechnology Letters ــ 32 (6). pp. 781-786. شاپا 1573-6776

Text - Published Version

Official URL: http://www.springerlink.com/content/r043463280786t...


Novel method to obtain highly enriched cultures of adult rat Schwann cells

English Abstract

Schwann cells (SCs) can be used to repair both the peripheral and central nervous systems. Therefore, establishment of a procedure to obtain activated, highly proliferative SCs, in an appropriate time for clinical applications, is a prerequisite. Purification is complicated by contamination with fibroblasts which often become the predominant cell type in an in vitro SC culture. This study describes a novel and efficient method to enrich SCs by utilizing the differential detachment properties of the two cell types. In culture, cells were treated with two different media and the chelator, EGTA, which detached SCs faster than fibroblasts and allowed for easy isolation of SCs. Within seven days, high yields of SCs with a purity of greater than 99% were achieved. This was confirmed by immunostaining characterization and flow-cytometric analyses using an antibody against the p75 low affinity nerve growth factor receptor (p75LNGFR). The entire procedure was completed in approximately 21 days. This method has the advantage of being technically easier, faster, and more efficient than other previously described methods. An SC culture that was about 99% homogenous was achieved.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :علی نیاپور
نویسنده مسئول :محمد حسين نصر اصفهاني
نویسنده مسئول :حسين بهاروند
Additional Information:Impact Factor(2010):1.768 Abstracted/Indexed in: 1-PubMed/Medline,Science Citation Index, Science Citation Index Expanded (SciSearch), 2- BIOSIS, Current Contents/ Life Sciences, EMBASE, SCOPUS, 3-Academic OneFile, AGRICOLA, ASFA, Biological Abstracts ,Biotechnology Citation Index, CAB Abstracts, CAB International, CEABA-VtB, Chemical Abstracts Service (CAS), CSA, Current Abstracts, Current Contents/ Agriculture, Biology & Environmental Sciences, EBSCO, EI-Compendex, Elsevier Biobase,EMBiology, Environment Index, Expanded Academic, Food Science and Technology Abstracts, GeoRef, Global Health, Google Scholar, Health Reference Center Academic, IBIDS, Index Copernicus, INIS Atomindex, Journal Citation Reports/Science Edition, OCLC, PASCAL,Reaction Citation Index, Summon by Serial Solutions, VINITI - Russian Academy of Science : Cited By Whitlon, D.S. (2010) Purification and transfection of cochlear Schwann cells. Neuroscience 171(1
کلیدواژه ها (انگلیسی):Sciatic nerve , Predegeneration, Schwann cell, Detachment property, Purification
Subjects:QS Human Anatomy
QU Biochemistry
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:2665
Deposited By: Dr Ali Niapour
Deposited On:10 Jul 1390 07:59
Last Modified:12 Nov 1391 10:05

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