جداسازی و کشت اولیه مناطق سری و دمی لوله عصبی در حال تکوین جنین جوجه

Title

isolation and primary culture of rostral and caudal zone of the chick developing neural tube

English Abstract

Background and goal: The cephalic and caudal zone of developing neural tube comprises neural precursor cells differentiating into neurons.The aim of this study was primary culture of the rostral and caudal zone upon isolation from chick embryo and in vitro differentiation into neuron. Materials & Methods : The rostral and caudal-most part of the developing neural tube were isolated from chick embryo satged %-16 according to Hamburger-Hamilton developmental table and cultured in 24-well dishes containing DMEM/F121Glutamax medium 1151 FBS which supplemnted by 11 Pen/Strep, 11NEAA, 1 mM 2-mercaptoethanol. Results: Neural – like cells started to project from cultured caudal zone after 4 days plating and produced a neural network around it up to 11 days post-plating. Interestingly, the neural rosette-like structure has been found to form in cultured telencephalon after 15 days which differentiated into neural tube-like structure showing a prominent lumen surrounded by neuroepuithelium upon 25 post-plating. Conclusion: The developing neural tube can differentiate into neural cells and rosette –like structures upon isolation from chick embryo and makes an in vitro model system for further investigation on neurvous system.

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