نیاپور, علی and کرمعلی, فرشته and نعمتی, شیوا and تقی پور, زهرا and مردانی, محمد and نصر اصفهاني, محمد حسين and بهاروند, حسين (1391) پیوند همزمان سلول های پیش ساز عصبی مشتق از سلول بنیادی جنینی انسانی و سلول های شوان درآسیب طناب نخاعی موش صحرايى از نوع کوفتگی، سبب افزایش مشخص تمایز پیش ساز عصبی به سمت نورون و بهبودی حرکتی حیوان می گردد. Cell transplantation ــ 21 (5). pp. 827-843. شاپا 0963-6897
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Title
Co-transplantation of Human Embryonic Stem Cell-derived Neural Progenitors and Schwann Cells in a Rat Spinal Cord Contusion Injury Model Elicits a Distinct Neurogenesis and Functional Recovery
English Abstract
Co-transplantation of neural progenitors (NPs) with Schwann cells (SCs) might be a way to overcome low rate of neuronal differentiation of NPs following transplantation in spinal cord injury (SCI) and the improvement of locomotor recovery. In this study, we initially generated NPs from human embryonic stem cells (hESCs) and investigated their potential for neuronal differentiation and functional recovery when co-cultured with SCs in vitro and co-transplanted in a rat acute model of contused SCI. Co-cultivation results revealed that the presence of SCs provided a consistent status for hESC-NPs and recharged their neural differentiation toward a predominantly neuronal fate. Following transplantation, a significant functional recovery was observed in all engrafted groups (NPs, SCs, NPs+SCs) relative to the vehicle and control groups. We also observed that animals receiving co-transplants established a better state as assessed with the BBB functional test. Immunohistofluorescence evaluation five weeks after transplantation showed invigorated neuronal differentiation and limited proliferation in the co-transplanted group when compared to the individual hESC-NPs grafted group. These findings have demonstrated that the co-transplantation of SCs with hESC-NPs could offer a synergistic effect, promoting neuronal differentiation and functional recovery.
| Item Type: | Article |
|---|---|
| زبان سند : | انگلیسی |
| نویسنده اول : | علی نیاپور |
| نویسنده مسئول : | محمد حسين نصر اصفهاني |
| نویسنده مسئول : | حسين بهاروند |
| Additional Information: | Impact Factor: (2012) 4.422 Indexed in: ISI, Scopus, CABI , Ebsco, PROQUEST |
| کلیدواژه ها (انگلیسی): | Rat Schwann cell, Human neural progenitor, Co-culture, Co-transplantation, Differentiation, Spinal cord injury |
| Subjects: | QS Human Anatomy WL Nervous system |
| Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Anatomy |
| ID Code: | 3507 |
| Deposited By: | Dr Ali Niapour |
| Deposited On: | 20 Jun 1391 08:38 |
| Last Modified: | 05 Aug 1394 08:50 |
Available Versions of this Item
- پیوند همزمان سلول های پیش ساز عصبی مشتق از سلول بنیادی جنینی انسانی و سلول های شوان درآسیب طناب نخاعی موش صحرايى از نوع کوفتگی، سبب افزایش مشخص تمایز پیش ساز عصبی به سمت نورون و بهبودی حرکتی حیوان می گردد. (deposited 20 Jun 1391 08:38) [Currently Displayed]
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