مطالعات غیرفعال سازی و قفل کنفورماسیونی گلوکز اکسیداز

ستاری, زهرا and پور فیض, حسین and دهقان, غلامحسین and امانی, مجتبی and موسوی موحدی, علی اکبر (1391) مطالعات غیرفعال سازی و قفل کنفورماسیونی گلوکز اکسیداز. Structural Chemistry ــ . pp. 1-6. شاپا 1040-0400

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Official URL: http://link.springer.com/article/10.1007%2Fs11224-...


Thermal inactivation and conformational lock studies on glucose oxidase

English Abstract

In this study, the dissociative thermal inactivation and conformational lock theories are applied for the homodimeric enzyme glucose oxidase (GOD) in order to analyze its structure. For this purpose, the rate of activity reduction of glucose oxidase is studied at various temperatures using b-D-glucose as the substrate by incubation of enzyme at various temperatures in the wide range between 40 and 70 �C using UV–Vis spectrophotometry. It was observed that in the two ranges of temperatures, the enzyme has two different forms. In relatively low temperatures, the enzyme is in its dimeric state and has normal activity. In high temperatures, the activity almost disappears and it aggregates. The above achievements are confirmed by dynamic light scattering. The experimental parameter ‘‘n’’ as the obvious number of conformational locks at the dimer interface of glucose oxidase is obtained by kinetic data, and the value is near to two. To confirm the above results, the X-ray crystallography structure of the enzyme, GOD (pdb, 1gal), was also studied. The secondary and tertiary structures of the enzyme to track the thermal inactivation were studied by circular dichroism and fluorescence spectroscopy, respectively. We proposed a mechanism model for thermal inactivation of GOD based on the absence of the monomeric form of the enzyme by circular dichroism and fluorescence spectroscopy.

Item Type:Article
زبان سند : انگلیسی
دانشجو :زهرا ستاری
استاد راهنما :حسین پور فیض
استاد مشاور :غلامحسین دهقان
استاد مشاور :مجتبی امانی
استاد راهنما :علی اکبر موسوی موحدی
Additional Information:Impact Factor: 1.846 Abstracted/Indexed in: Science Citation Index, Science Citation Index Expanded (SciSearch), Journal Citation Reports/Science Edition, SCOPUS, Chemical Abstracts Service (CAS), Google Scholar, EBSCO, Academic OneFile, Chemistry Citation Index, ChemWeb, Chimica, Current Abstracts, Current Contents/Physical, Chemical and Earth Sciences, EMBiology, Gale, OCLC, Reaxys, SCImago, Summon by Serial Solutions, VINITI - Russian Academy of Science
کلیدواژه ها (انگلیسی):Glucose oxidase , Conformational lock , Thermal inactivation , Dimeric form , Monomeric form ,Dynamic light scattering
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:3611
Deposited By: Dr Mojtaba Amani
Deposited On:04 Aug 1391 11:55
Last Modified:20 Jan 1393 12:35

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