مهار آنزیم امین اکسیداز جوانه نخود توسط تترااتیلن پنتامین

طلایی, سونیا and اسدی, اسداله and امانی, مجتبی (1391) مهار آنزیم امین اکسیداز جوانه نخود توسط تترااتیلن پنتامین. Molecular Biology Research Communications (MBRC) ــ 1 (1). pp. 27-32. شاپا 2322-181X

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Official URL: http://mbrc.shirazu.ac.ir/?_action=articleInfo&art...


Inhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine

English Abstract

Copper amine oxidases are important enzymes, which contribute to the regulation of monoand polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to molecular oxygen which is reduced to hydrogen peroxide. Inhibitors are important tools in the study of catalytic properties of copper amine oxidases and they also have a wide application in physiological research. In this study, purification of the chickpea seedling amine oxidase, was done via salting out by ammonium sulfate and dialysis, followed by DEAE-cellulose column chromatography. By using the Lineweaver - Burk plot, the Km and Vm of the enzyme were found to be 3.3 mM and 0.95 mmol/min/mg, respectively. In this study, the interaction of chickpea diamino oxidase with tetraethylene- pentamine was studied. Analysis of kinetic data indicated that tetraethylenepentamine (with Ki=0.1 mM) inhibits the enzyme by linear mixed inhibitory effect.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سونیا طلایی
نویسنده مسئول :اسداله اسدی
نویسنده :مجتبی امانی
Additional Information:Indexed: Index Copernicus ,JournalTOCs , Electronic Journals Library (EZB) , Cancer Research Library Online (CaRLO) ,SHERPA/RoMEO ,ResearchBib ,Virtual Library of Biology (vifabio), Electronic Journals (e.journals) Electronic Journals (WZB), Public Knowledge Project (PKP) NewJour ,Free Journals Act ,Academic Keys ,Undergoing in other international databases ,
کلیدواژه ها (انگلیسی):Chickpea; Copper-containing amine oxidases; Tetraethylenepentamine; Linear mixed
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:3760
Deposited By: Dr Mojtaba Amani
Deposited On:06 Sep 1391 13:52
Last Modified:18 Oct 1398 09:21

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