پیش بینی نفوذ آنتی بیوتیکی با حضور برخی از ژن β-لاکتاماز در اسینتوباکتر بیماران مبتلا به عفونت بستری در تهران

Title

Prediction of Antibiotic Influence by Presence of Some β-lactamase Gene in AcinetobacterInfected Patients Hospitalized in Tehran, Iran

English Abstract

Acinetobacter baumannii which is gram-negative rod and infecting hospitalized patient often develops multiple drug resistance patterns and causes serious problem in treatment. The aim of this study is to determine the relationship between this serious problem in treatment and Presence of some β-lactamase genes in the A. baumannii. 100 isolates of Acinetobacter collected from hospitalized patients in Tehran. First their resistance to different antibiotics were determined using disk agar diffusion test then the MICs of all resistant isolates determined by Etest .In the second stage we screened all resistance isolates for β-lactamase gene existence by double-disk synergy test and finally prevalence of β-lactamase encoding genes such as IMP-1,SIM-1,OXA-23,OXA-24 and OXA-58 were determined by PCR. According those tests the most efficient treatment was achieved by Colistin, Gentamicin, and Imipenem. Disk synergy test with 0.1M EDTA determined 23 % of isolates as metalo β-lactamase producers. Prevalence of β- lactamase encoding genes was: 4%, 0%, 38%, 32%, and 1% respectively. According to this study efficient treatment will be influenced by OXA β-lactamase more than the other genes which have been studied. This is the first report of SIM type among MBL-producing A. baumannii in Iran.

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