غیرفعال سازی و قفلهای کنفورماسیونی کربونیک انهبدراز گاوی

علایی, لقمان and موسوی موحدی, علی اکبر and هادی علیجانوند, حمید and صبوری, علی اکبر and احمد, فیضان and امانی, مجتبی (1391) غیرفعال سازی و قفلهای کنفورماسیونی کربونیک انهبدراز گاوی. Protein and Peptide Letters ــ 19 (8). pp. 852-858. شاپا 0929-8665

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Thermal Inactivation and Conformational Lock of Bovine Carbonic Anhydrase

English Abstract

The kinetics of thermal inactivation of bovine carbonic anhydrase (BCA) was studied in a 50 mM Tris-HCl buffer, pH 7.8 using p-nitrophenyl acetate as substrate in absorbance of 400 nm by UV-VIS spectrophotometry. The number of conformational locks and inter-subunit amino acid residues of BCA were obtained by thermal inactivation analysis. The cleavage bonds between dimers of BCA during thermal dissociation and type of interactions between specific amino acid residues were also detected. The thermal inactivation curves were plotted in temperatures ranging between 40-70°C. It was shown several phases for inactivation of BCA at 65°C. Analyses of the curves were done by the conformational lock theory. The subunits are dissociated and several intermediates appear during inactivation through increasing the temperature in comparison with native state. Dynamic light scattering measurements was done to study the changes in hydrodynamic radius during thermal inactivation. Three distinct zones were shown in DLS data. Biochemical computation using ligplot is performed to find the inter-subunit amino acid residues for BCA

Item Type:Article
زبان سند : انگلیسی
دانشجو :لقمان علایی
نویسنده مسئول :علی اکبر موسوی موحدی
نویسنده :حمید هادی علیجانوند
نویسنده :علی اکبر صبوری
نویسنده :فیضان احمد
نویسنده :مجتبی امانی
Additional Information:Impact Factor : 1.942 Indexed: 1-Science Citation Index Expanded 2- BIOSIS Previews PubMed - MEDLINE, ndex Medicus
کلیدواژه ها (انگلیسی):Carbonic anhydrase, kinetics, conformational lock, thermal inactivation, intersubunit interactions
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:3875
Deposited By: Dr Mojtaba Amani
Deposited On:20 Oct 1391 09:34
Last Modified:20 Jan 1393 12:38

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