title

تشخیص بروسلوز انسانی با استفاده از محیط کشت BACTEC و روشهای PCR خون و سرم

علیخانی, محمد یوسف and هاشمی , سید حمید and ناصری, زهرا and فرج نیا, صفر and پیری دوگاهه, هادی (1392) تشخیص بروسلوز انسانی با استفاده از محیط کشت BACTEC و روشهای PCR خون و سرم. jundishapur journal of microbiology ــ 6 (3). pp. 248-251. شاپا 2008-4161

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Title

Diagnosis of Human Brucellosis by Blood Culture (BACTEC) and PCR Method via Whole Blood and Serum

English Abstract

Brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including Iran. Diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. Rapid detection of Brucella species by an automated blood culture system and Polymerase Chain Reaction (PCR) may lead to an earlier diagnosis and may improve patient management. Objectives: The current study aimed to evaluate PCR technique as a diagnostic tool for brucellosis in comparison to conventional bacteriological techniques. Materials and Methods: A total of 50 patients suspected to have brucellosis were included in this study. All patients presented clinical signs compatible with brucellosis. Diagnosis was established by detecting a titer equal to or greater than 1:160 by the standard tube agglutination (STA) method. Blood samples and sera from the patients were tested by culture using BACTEC 9050 system and PCR using primer set to amplify a 223 bp region with in the gene coding for a 31 KD Brucella antigen. Results: Eleven (22%) whole blood samples and 17 (34%) serum samples out of 50 had positive PCR and 7 (14%) patients had Brucella species grown in their cultures. Out of 43 blood culture negative samples, 10 (23.3 %) were positive with the serum PCR versus in 4(9.3 %) with whole blood PCR. Conclusions: The results suggest that the serum PCR assay is rapid and easy to perform and highly sensitive and specific, and it may therefore be considered a useful tool for diagnosis of human brucellosi

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمد یوسف علیخانی
نویسنده مسئول :سید حمید هاشمی
نویسنده :هادی پیری دوگاهه
Additional Information:Impact factor: 0.474 ISI Web of Knowledge, Science Citation Index Expanded, SCOPUS, EMBASE, EBSCO, CABI, ProQuest, Elsevier Bibliographics Databases, Scirus (Elsevier), U.S. National Library of Medicine (NLM), DOAJ, E-Journals, EMRmedex, WHO Hinari, Index Copernicus, Google Scholar, UDL Theses (Knowledge from books to bytes), Ulrichs WEB (Global serials directory), GFMER (Geneva Foundation for Medical Education and Research), Open J Gate, ResearchGATE, Scimagojr, Academic Journals Database ISC, SID, Iranmedex, Iranhivd, Iranenvex, Magiran, MEDLIB
کلیدواژه ها (انگلیسی):Brucellosis; Polymerase Chain Reaction; Blood; Culture
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:4207
Deposited By: Dr Hadi Peeridogaheh
Deposited On:30 Feb 1392 13:02
Last Modified:05 Mar 1392 10:26

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