افزایش بیان گیرنده اسید رتینوئیک در سلولهای بنیادی مزانشیمی مشتق از بندناف انسان

سرایی, فرنوش and محسنی کوچصفهانی, هما and ملکی, مسعود and سقا, محسن (1392) افزایش بیان گیرنده اسید رتینوئیک در سلولهای بنیادی مزانشیمی مشتق از بندناف انسان. در: 1st Tabriz International Life Science Conference & 12th Iran Biophysical Chemistry Conference (TILSC & IBCC) 22-24 May, 2013, Tabriz, Iran., 1-3 خرداد 92 , تبریز.

Text - Published Version

Official URL: http://faobmb.net/p4-meetings-1a.asp?y=2013&n=9


Retinoic Acid Receptor Overexpression in Human Umbilical Cord-derived Mesenchymal Stem Cells

English Abstract

Introduction: Retinoic acid (RA) involves in vertebrate morphogenesis, growth and apoptosis through two classes of receptors encoded by six genes; RAR,   and RXR, . The former utilizes either all-trans RA or 9-cis-RA as ligands, whereas the RXRs utilize only 9-cis-RA. Using the human umbilical cord derived stem cells (HUCSCs) as an in vitro model of human fetal cells we aimed to evaluate RAR overexpression following to RA treatment. Methods: HUCSCs were cultured in DMEM + 10% FBS at a density of 1 × 103/ well. Upon adhering, the medium was changed to DMEM containing RA for 4-6 days during which RA refreshed every 2 days. The untreated cells were considered as a control group. Using a combination of flowcytometry, MTT colorimetric assay and conventional RT-PCR techniques, CD markers, cell viability and RAR expression profile of HUCSCs were measured, respectively. Results: Flowcytometry analysis clearly indicated 5.4% of HUCSCs co-expressed CD34 and CD45, while 63.7% of cells expressed both CD44 and CD73. 36.5% of cells expressed CD90 compared to 0.05% for CD105.MTT assay also showed that about 60% of HUCSCsˈ viability decreased at higher doses (10-7 – 10-5M) of RA compared to control group. RT-PCR analysis also revealed that RAR  and were upregulated in the RA–treated cells. Conclusions: This study clearly shows that the HUCSCs express CD44, CD73 and CD90 and RA in a dose-dependent manner has a cytotoxicity effect on HUCSCs that is mediated by RAR  and 

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
دانشجو :فرنوش سرایی
نویسنده :هما محسنی کوچصفهانی
نویسنده :مسعود ملکی
نویسنده مسئول :محسن سقا
کلیدواژه ها (انگلیسی):Human Umbilical Cord-derived mesenchymal Stem Cells, RAR, RXR, Retinoic acid, Cytotoxicity
Subjects:QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:4271
Deposited By: Dr Mohsen Sagha
Deposited On:13 Mar 1392 12:42
Last Modified:11 Oct 1392 10:10

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