افزایش بیان گیرنده اسید رتینوئیک در سلولهای بنیادی مزانشیمی مشتق از بندناف انسان

Title

Retinoic Acid Receptor Overexpression in Human Umbilical Cord-derived Mesenchymal Stem Cells

English Abstract

Introduction: Retinoic acid (RA) involves in vertebrate morphogenesis, growth and apoptosis through two classes of receptors encoded by six genes; RAR,   and RXR, . The former utilizes either all-trans RA or 9-cis-RA as ligands, whereas the RXRs utilize only 9-cis-RA. Using the human umbilical cord derived stem cells (HUCSCs) as an in vitro model of human fetal cells we aimed to evaluate RAR overexpression following to RA treatment. Methods: HUCSCs were cultured in DMEM + 10% FBS at a density of 1 × 103/ well. Upon adhering, the medium was changed to DMEM containing RA for 4-6 days during which RA refreshed every 2 days. The untreated cells were considered as a control group. Using a combination of flowcytometry, MTT colorimetric assay and conventional RT-PCR techniques, CD markers, cell viability and RAR expression profile of HUCSCs were measured, respectively. Results: Flowcytometry analysis clearly indicated 5.4% of HUCSCs co-expressed CD34 and CD45, while 63.7% of cells expressed both CD44 and CD73. 36.5% of cells expressed CD90 compared to 0.05% for CD105.MTT assay also showed that about 60% of HUCSCsˈ viability decreased at higher doses (10-7 – 10-5M) of RA compared to control group. RT-PCR analysis also revealed that RAR  and were upregulated in the RA–treated cells. Conclusions: This study clearly shows that the HUCSCs express CD44, CD73 and CD90 and RA in a dose-dependent manner has a cytotoxicity effect on HUCSCs that is mediated by RAR  and 

Document Downloads