پیگیری سیتوژنتیکی و ملکولی بر روی 259 بیمار با پیوند مغز استخوان در ایران

مهدی پور, پروین and قوام زاده, اردشیر and جهانی, محمد and ایروانی, مسعود and اخوان, هما and حسینی اصل, سید سعید and خدابنده, امیر and علی مقدم, کامران and بهار, بابک and بایبردی, ای and Abbasi, Manahil Andleeb and نجفی, بهروز and محی الدین, ماندانا (1381) پیگیری سیتوژنتیکی و ملکولی بر روی 259 بیمار با پیوند مغز استخوان در ایران. در: 2nd Internationaln Congress on Advances in Hematology-Oncology and BMT, September 25-29, 2002, Tehran - Iran.



Cytogenetic and molecular follow-up study on 259 bone marrow transplanted patients in Iran

English Abstract

Introduction: Bone marrow transplantation (BMT)is the treatment of choice for many patients with poor prognosis, relapsed or refractory leukemia who have matched donors, and one the attractive points in hematopoietic studies is to investigate chimerism. Patients and Methods: Investigation was carried out in 259 patients at diagnosis. The most frequent disease included thalassemia (88), AML (53), CML (30), ALL (16) and others (72). The cytogenetic study was carried out on BM-cells and/or PB-lymphocytes according to the standard culture procedures and GTG. In some cases, FISH and molecular investigation on 4 exons of P53 gene, by PCR-SSCP and heteroduplex analysis techniques were carried out. Results and Conclusion: Cytogenetic investigation was focused on 190 patients at diagnosis (Pre-BMT) and 120 patients at post-BMT. Among donors, 97.9% were first-degree relatives (58.3% brother and 39.6% sister). In CML-patients 93.4% were philadelphia-positive. The most frequent chromosome alterations were: (1) In thalassemia-patients, in pre-BMT small 1qh(20%) and 9qh+ (20%), as polymorphisms and del (1)(q13), del (4)(13) and del (10) and in post-BMT: gains of X (14.6%), Y (10.6%), ... . the preliminary data on mutation detection of 4 exons in P53 gene revealed 10% shifts by application of the dual techniques including PCR-SSCP and heteroduplex analysis. The present data could lead us to plan a multidisciplinary investigation, including cytogenetic and molecular genetics for the BMT-patients.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده مسئول :پروین مهدی پور
نویسنده :اردشیر قوام زاده
نویسنده :محمد جهانی
نویسنده :مسعود ایروانی
نویسنده :هما اخوان
نویسنده :سید سعید حسینی اصل
نویسنده :امیر خدابنده
نویسنده :کامران علی مقدم
نویسنده :بابک بهار
نویسنده :ای بایبردی
نویسنده :Manahil Andleeb Abbasi
نویسنده :بهروز نجفی
نویسنده :ماندانا محی الدین
کلیدواژه ها (انگلیسی):Cytogenetic , Bone marrow transplantation , PCR
Subjects:QY Clinical Pathology
WE Musculoskeletal system
WO Surgery
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Genetics
ID Code:4412
Deposited By: Dr S Saied Hosseini-Asl
Deposited On:22 May 1392 11:08
Last Modified:01 Dec 1402 13:42

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