شیوع جهش های p53 در بیماری هوچکین

صدرزاده, امیر حسین and رفیعی, رضا and رحمانیان, مسعود and حسینی اصل, سید سعید and شهبازی, مجید and صادقی فر, امیر and کیهانی, منوچهر and جهانزاد, عیسی and مهدی پور, پروین (1382) شیوع جهش های p53 در بیماری هوچکین. در: the First International Congress of Cancer Genetics, 13-16 Dec, 2003, Tehran - Iran.



P53 gene mutations prevalence in Hodgkin's disease

English Abstract

p53 tumor-suppressor gene which encodes a nuclear phosphoprotein that arrests cell cycle progress at G1, is one of the most frequently mutated genes in human cancers. In this study, 40 cases of Hodgkin's disease (HD) which is the most common lymphoma in young adults, have been investigated for P53 gene mutations. For each case, sections of paraffin-embedded tissue blocks were cut, a light hematoxylene stain was applied, and in order to enrich our samples for a higher percentage of Reed-Sternberg (RS) cells which are thought to be the neoplastic component of HD lesions, a microdissection technique was used. We used polymerase chain reaction (PCR) amplification technique and single strand conformational polymorphism analysis (SSCP) to study mutations whithin exons 5 to 8 of gene P53. Seven shifts were detected in six cases (15%), identified by SSCP (one shift in exon 5A, one in exon 5B, one in exon 6, two in exon 7 and one in exon 8). Since the specificity of PCR-SSCP is more than 95% for 100 to 300 bp polymerase chain reaction fragments, it is conceivable that p53 gene mutation is not a common feature in HD. However, the samples with abnormal SSCP require nucleotide sequencing studies to assess whether the abnormal migration represents a mutation or a polymorphism.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :امیر حسین صدرزاده
نویسنده :رضا رفیعی
نویسنده :مسعود رحمانیان
نویسنده :سید سعید حسینی اصل
نویسنده :مجید شهبازی
نویسنده :امیر صادقی فر
نویسنده :منوچهر کیهانی
نویسنده :عیسی جهانزاد
نویسنده مسئول :پروین مهدی پور
کلیدواژه ها (انگلیسی):PCR , Gene , Hodgkin
Subjects:QY Clinical Pathology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Genetics
ID Code:4416
Deposited By: Dr S Saied Hosseini-Asl
Deposited On:22 May 1392 11:36
Last Modified:07 Jul 1393 05:30

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