اثر همادو بر سطح آنزیم های کراتین کیناز-ام بی و لاکتات دهیدروژناز بدنبال آسیب ایسکمی-ریپرفیوژن در قلب ایزوله رت

امانی, محمد and جدی, سجاد and احمدی اصل, ناصر and یوسف زاده, نصیبه and زمان, جلال (1392) اثر همادو بر سطح آنزیم های کراتین کیناز-ام بی و لاکتات دهیدروژناز بدنبال آسیب ایسکمی-ریپرفیوژن در قلب ایزوله رت. BioImpacts ــ 3 (2). pp. 101-104. شاپا 2228-5652

Text - Published Version

Official URL: http://bi.tbzmed.ac.ir/JournalIssues/AllIssues/Vol...


Effect of HEMADO on Level of CK-MB and LDH Enzymes after Ischemia/Reperfusion Injury in Isolated Rat Heart

English Abstract

Introduction: Ischemia/Reperfusion (IR) injury mainly causes the increase of enzymes involved in myocytes injury including CK-MB (creatine kinase-MB) isoenzyme and LDH (lactate dehydrogenase). Leakage of CK-MB isoenzyme and LDH from myocardial tissues to blood is indicator of acute myocardial infarction. The aim of this study was to assess the effect of HEMADO on IR injury and its relationship with mitochondrial ATP-sensitive K+ channels (mitoKATP) in rat heart. Methods: Twenty eight male Wistar rats (250-300g) were divided into four groups (seven members in each group): control (without ischemia), I/R (with ischemia+without HEMADO), ischemia received HEMADO (HEMADO), ischemia received HEMADO and 5-HD (5-hydroxydecanoate, specific mitoKATP channel blocker) (HEMADO+5-HD). The animals were anesthetized and the hearts were quickly removed and mounted on Langendorff apparatus and perfused by Krebs-Henseleit solution under constant pressure and temperature of 37ºC. After 20 minutes of stabilization, ischemic groups were exposed to 40 minutes of global ischemia and consecutive 90 minutes of reperfusion. Results: IR injury increased the level of LDH and CK-MB in the collected coronary flow during 5 minutes since start of reperfusion. HEMADO reduced the enzymes' levels and using 5-HD abolished the effect of HEMADO. Conclusion: Our findings indicated that HEMADO could protect the heart against ischemia-reperfusion injury by decreasing the CK-MB and LDH levels. The cardioprotective effect of HEMADO may be mediated in part by mitoKATP.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمد امانی
نویسنده مسئول :سجاد جدی
نویسنده :ناصر احمدی اصل
نویسنده :نصیبه یوسف زاده
نویسنده :جلال زمان
Additional Information:Indexing and Abstracting Services: PubMed, Chemical Abstracts Service , Scopus, PubMed Central, EBSCOhost , DOAJ: Directory of Open Access Journals Google Scholar , Index Copernicus , Islamic World Science Citation Center (ISC)
کلیدواژه ها (انگلیسی):HEMADO , Lactate Dehydrogenase , Creatine Kinase, Ischemia/Reperfusion Injury , mitoKATP
Subjects:QT physiology
QU Biochemistry
QV pharmacology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:4426
Deposited By: Dr Mohammad Amani
Deposited On:15 May 1392 10:28
Last Modified:10 Jan 1393 08:48

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