مطالعه شباهت ژنتیکی ژیاردیای گربه و انسان با روش PCR-RFLP

پزشکی, علی and زارع بوانی, میترا and رضائیان, مصطفی and رضائی, ساسان (1387) مطالعه شباهت ژنتیکی ژیاردیای گربه و انسان با روش PCR-RFLP. در: XVIIth International congress for Tropical Medicine and Malaria, September 29-October 3, 2008, Jeju , Korea.

Text - Published Version


The study of the genetic resemblance of cat and human Giardia with PCR-RFLP method

English Abstract

Giardia duodenalis is the most common intestinal parasite with cosmopolitan distribution. This parasite has been found in the intestine of humans and other mammalian hosts including cats, dogs, cattle, sheep, deer, pigs and muskrats. It is postulated that animals maybe reservoir for human infection and, vice versa. In present study, the possible genetic similarity between cat and humans Giardia and its probable zoonosis were investigated. Direct examination and formalin-ether concentration techniques were performed on stray and semi stray cat fecal specimens. Gradient sucrose method was applied for the collection and purification of cysts, and DNA extraction was performed by phenol-chloroform method. DNA of cysts could hardly be extracted after repeated freezing and thawing. Polymerase chain reaction (PCR) was performed for DNAamplification. In this study, triosephosphate isomarase (tpi) gene was selected as a molecular marker. Two sets of primers (PM290 and PM924) were considered. Two restriction enzyme RsaI and AvaI were also used to determine restriction fragment length polymorphism (RFLP) for PCR fragments amplified by both primer sets. Ten samples from 166 samples were positive for Giardia cysts which were examined for molecular investigation. Four cat isolates were amplified by PM290. PCR-RFLP patterns were found to be similar to human ACAF069556 (subgroup of ACU57897) with possibility of cross-transmission. Therefore the similarity of genomic characters of isolates of cat and human Giardia implies possibility of zoonosis and transmission of these protozoa from cat to human and vice versa.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :علی پزشکی
نویسنده :میترا زارع بوانی
نویسنده :مصطفی رضائیان
نویسنده :ساسان رضائی
کلیدواژه ها (انگلیسی):PCR-RFLP , Giardia , Human , Cat
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:4505
Deposited By: Dr Ali Pezeshki
Deposited On:20 May 1392 09:38
Last Modified:20 May 1392 09:38

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