پاسخ سایتوکاینی Th-1 نسبت به پروتئین غشای خارجی 18کیلودالتونی (Omp19) نوترکیب بروسلا آبورتوس

ذاکرزاده, آرمان and خرم آبادی, نیما and آقابابا, هانیه and بختیاری, امیر and مجتبی مبارز, اشرف and نجاتی, مهدی and تبرایی, بهمن and بهمنش, مهرداد and آذرنیاد, زهرا and بهشتی, نازنین and خدادادی, صاینه (1391) پاسخ سایتوکاینی Th-1 نسبت به پروتئین غشای خارجی 18کیلودالتونی (Omp19) نوترکیب بروسلا آبورتوس. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Th-1 Type Cytokine Response to Recombinant 18kDa Outer Membrane Protein (Omp19) of Brucella abortus

English Abstract

Background & Objeactives: As a prevalent zoonose, brucellosis is of great concern economically. Attenuated vaccine strains are available for prevention of the disease in cattle but not in humans. Development of new efficient vaccines also useful for humans is a field of interest for researchers. Brucella antigens which induce cell-mediated immune response may have a key role in development of new vaccines. This study aimed to survey cytokine response to the recombinant protein moiety of 18kDa lipoprotein (Omp19) of brucella abortus. Methods: opm19 gene cloned in pET28a(+) and the recombinant Omp19 produced in E. coli Bl-21. Recombinant protein purified using Ni-NTA resin. Recombinant Omp19 administered subcutaneous to BALB/c mice emulsified with ferund’s adjuvant. Two boosters with twelve-day intervals administered to mice. Six day after last booster, mice sacrificed and spleen lymphocytes isolated and cultured. IL-4 and IFN-γ levels in response to stimulation with recombinant Omp19 assayed subsequently. Results: Lymphocyte from Omp19-immunized mice produced a higher level of IFN-γ compared to non-immune ones (p<0.001). No difference observed between immunized and non-immunized groups in IL-4 levels. Conclusion: Our results suggest that Brucella abortus Omp19 induces cell-mediated immune response in mice. Cell-mediated response is essential in battling brucellosis; thus Omp19 is a suitable antigen in new vaccine investigations. Brucellosis, Brucella abortus, Omp19, IL-4, IFN-γ

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :آرمان ذاکرزاده
کلیدواژه ها (انگلیسی):Brucellosis ; Brucella abortus ; Omp19 , IL-4 ; IFN-γ
Subjects:QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4558
Deposited By: MS Soghra Golmaghani
Deposited On:05 Jun 1392 11:01
Last Modified:10 Jan 1393 10:18

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