بررسی اپیدمیولوژی مولکولی استافیلوکوکوس اورئوس های واجد ژن ACME_arcA در افراد ناقل سالم

Title

Molecular Epidemiology of Staphylococcus aureus Carriage ACME-arcA Gene in Healthy Carrier

English Abstract

Background & Objectives: Staphylococcus aureus continues to be an important human pathogen in both healthcare and community settings. The arc gene cluster within the arginine catabolic mobile element ACME may has function as a virulence or strain survival factor. This study aimed to determine molecular epidemiology and prevalence of the ACME-associated arcA gene among S.aureus isolated from healthy student of Arak University of Medical Sciences. Methods:From the 568 nasal swap specimens , 82 S.aureus isolated by S. aureus–specific isolation procedures. Isolates thatcarriage ACME-arcA gene were identified by PCR, as well as PCR for determination ofSingle-locus spa sequence typing was carried out, then Data were analysed using Ridom StaphType software. Results: Among 82 S.aureus isolates, 11 (13.4%) were positive for arcA gene. Strain with spa type t660 was the only Methicillin-resistant Staphylococcus aureus (MRSA) and spa type t1149, t074, t790, t002, t021, t 084, t4892, t3204, t701(two isolates) were known as Methicillin-susceptible Staphylococcus aureus (MSSA). Conclusion:The detection of ACME-arcA in diverse S.aureus types highlights the mobility of the elements encoding ACME-arcA genes.The diversity of strain types among ACME-arcA-encoding S.aureus is a cause for public-health concern and demands continued surveillance and close monitoring.

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