ارزیابی روشهای مطمئن برای جداسازی هلیکوباکتر پیلوری در بیماران مبتلا به اختلالات دستگاه گوارش

عنوان انگليسی

Evaluation of Reliability of Stool Culture Methods for Isolation of Helicobacter pylori in Symptomatic Adolescence Patients with Gastrointestinal Disorders

خلاصه انگلیسی

Background & Objectives: Helicobacter pylori recognized as the main etiological agent of peptic ulcers and chronic gastritis.Fecal-oral is recommended as one of the probable routs of its spreading; it can be proved when active forms of bacterium isolate from feces of infected people but there are only a few successesful attempts. Our main goal was that, too. Methods: From total 98 symptomatic adult patients have been referred to the endoscopy ward of motahary clinic in shiraz-Iran, antral biopsies were taken for Rut, culture and histopathology visualization and fresh stool gathered from each one simultaneously were cultivated for isolation of H.pylori by two culture protocols after 24, 48 and 72 hours. First methods with trimetoprime and second with cholestyramine treatment. All suspected H.pylori colonies checked with biochemical H.pylori identification tests. Final confirmations were done with multiplex PCR methods. Results: After 24 hoursby the first Methods 66% of manual inoculated H. pylori could not convert to cultivable form; it determined 34.5% by the second methods but after 48 hours 50% and 22% was estimated, by two methods respectively. It was 47% and 22% after 72 hours. The highest isolation was 56% by cholestyramine treatment, after 48 hours. By the second methods revival amount of viable forms were equal at 48 and 72 hours (78%) but after 72 hours active forms shifted to coccoid shapes. But, in vivo, we could not isolate any vital H. pylori in no way. Conclusion: Although our culturing results on controlling step by adding vital form of H. pylori to the feces of non patients illustrated cholestyramine improves recovery of H. pylori from stool, but in vivo, H. pylori were not isolated at all. We concluded during passing H. pylori through feces in adults, there are many degradative agents causing loss of H. pylori viability so it finally could not be cultivate although could be tracked by PCR or Ag detection analysis. So cholestyramine only could improve the yield of H. pylori from stool when morphological shifts occurred from spiral shape to coccoid form.

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