توانایی تجزیه زیستی فنل توسط دو جدایه باکتریایی از خاک زراعی

نافیان دهکردی, فاطمه and غروی, سارا and صعودی, محمدرضا (1391) توانایی تجزیه زیستی فنل توسط دو جدایه باکتریایی از خاک زراعی. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Phenol Biodegradation by Two Bacterial Isolates From Agricultural Soil

English Abstract

Background & Objectives: Aromatic compounds, with toxic effects on most organisms, are the largest category of materials resistant to degradation. For microorganisms however, the biodegradation of these compounds goes through the carbon cycle. Although, genetic engineering techniques are currently utilized to enhance the abilities of bacteria for bioremediation, the biochemical potentials of the microorganism should first be established. In this study, the ability of phenol biodegradation was investigated by two isolated bacteria from agricultural soil samples. Methods: The GSN3 and GSN5 isolates were inoculated in a mineral culture consisting of 500 mg/l phenol as the sole carbon source and energy. Growth was monitored by optical density measurements at 600nm every 12 h and was followed up to 72 h. The GSN3 and GSN5 isolates reached the highest OD 0.8 and 0.7, respectively, after 14 hours and after 72 hours. The OD rates were assessed at concentrations of 1000 and 2000mg / l phenol after 72 hours. Results: The OD of culture broth of GSN3 and GSN5 isolates was 1.45 and 0.98, at a concentration of 1000mg / l phenol respectively, and was 0.19 and 0.17 at the concentration of 2000mg /L of phenol, respectively. Based on the increase in growth kinetics, an estimate of phenol degradation by the two isolates in the media was calculated and shown to be promising. However, further investigation is required to quantitatively evaluate the potential of the isolated bacteria vis-à-vis phenol bioremediation.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :فاطمه نافیان دهکردی
کلیدواژه ها (انگلیسی):Bioremediation ; Phenol Biodegradation ; Spectrophotometery
Subjects:WA Public Health > WA 30 Social, Economic, and Environmental factor in public health
WA Public Health > WA 30 Social, Economic, and Environmental factor in public health

QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4571
Deposited By: MS Soghra Golmaghani
Deposited On:06 Jun 1392 12:09
Last Modified:06 Jun 1392 12:09

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