مقایسه انکپسولاسيون غلظت های مختلف آنتی ژن لپتوسپيرا در ميکروسفرهای آلژينات به منظور تهیه واکسن لپتوسپیروز

Title

A Comparison between Encapsulation Efficiency in Different Concenteration of Leptospiral Antigen in Alginate Microsphere for New Leptospirosis Vaccine

English Abstract

Background & Objectives: Leptospirosis is a world-wide zoonosis caused by the genus Leptospira. Pathogenic leptospires are important primarily for their ability to cause disease in human and animals. Leptospirosis was described as a characteristic febrile jaundice of human (weil's disease) during latter oart of the nineteenth century. Leptospiral vaccine, (Leptospira grippotyphosa, Leptospira sergiohardjo, Leptospira canicola) has been used as effective vaccine to elicit specific antibodies for protection. Vaccine failed to induce long-lasting immunity. Thus, there is a need of more effective vaccine that not only elicits immunity across the heterologous Leptospira spp. serovars but also induces long-lasting immunological memory. In this research it has been tried to have a comparison encapsulation in different concenteration of leptospiral antigen in alginate microsphere to choose the best antigen concentration for new leptospiral vaccine. Methods: Best formulation for preparation of microsphere with SEM and particle sizer had been selected, then LA including 3 serovar (whole-killed cell) with different concentrations were loaded into microspheres. Encapsulation of each concentration had been calculated and the best result will be used in animal experiments. Results: Encapsulation efficiency of leptospiral antigen with these concentration 5, 15, 15 mg/ml are: 80%, 95.3% and 98.5%. Therefore highest concentration of LA (leptospiral antigen), have the highest encapsulation efficiency and loading capacity. Conclusion: To prepare a better vaccine for leptospirosis, encapsulated antigen could be used. In this study the best concentration of LA with the highest encapsulation had been selected that with more in-vitro and animal tests, probably it could be used as a new vaccine.

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