شیوع بالای ژن های مقاومت به آمینوگلیکوزیدها در جدایه های استافیلوکوکوس اورئوس جدا شده از یک ICU در تهران

سیدجوادی, سیما سادات and آل بویه, مسعود and ناظم الحسینی مجرد, احسان and زالی, محمدرضا (1391) شیوع بالای ژن های مقاومت به آمینوگلیکوزیدها در جدایه های استافیلوکوکوس اورئوس جدا شده از یک ICU در تهران. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version


High Prevalence of Genes Encoding Main Aminoglycoside-Modifying Enzymes in Isolates of Staphylococcus aureus in an ICU in Tehran

English Abstract

Background & Objectives: S. aureus in particular methicillin-resistant S. aureus (MRSA) are increasingly important causes of hospital-and community acquired infections throughout the world. Resistance against aminoglycosides (gentamicim and tobramycin), which act synergistically with beta lactams and glycopeptides,among S. aureus strains is more important clinically. Aminoglycoside-Modifying Enzymes (AME ), AAC (6')-APH(2'')], ,APH(3')-III and ANT(4')-I encoded by the aac(6')-Ie- aph(2'), aph(3')-III and ant(4')-I genes, are involve in this resistance. Despite the high incidence of aminoglycoside resistance to staphylococci, especially among MRSA, there is currently little information on the incidence and types of AMEs in many countries.The aim of the present study is to provide information regarding the prevalence of AMEs among Staphylococcus isolates obtained from a hospital in Tehran. Methods: Between August 2011 to March 2012, 108 S. aureus isolates were obtained from clinical and environmental samples of an ICU in Tehran, Iran. The isolates were confirmed by standard bacteriological methods. Testing for susceptibility to the antibiotics was performed using a standard disk-diffusion Methods according to the CLSI guidelines. All S. aureus isolates were examined by PCR for the aac(6')-Ie- aph(2'), aph(3')-III and ant(4')-I genes, using specific primers. Results: Out of 476(83%) environmental and 96(70.5%) clinical samples, S. aureus was detected in a frequency of 80 (17%) and 28 (29.5%), respectively. Methicillin-susceptible Staphylococci were more susceptible to aminoglycoside antibiotics than the methicillin-resistant Staphylococci. More than 90% of MRSA isolates were resistant to kanamycin, tobramycin and gentamicin.The most prevalent gene was aac(6')-Ie- aph(2'),had been found in 92.5%of the isolates, followed by ant(4')-I and aph(3')-III, found in 87% and 81.4% of these isolates, respectively. In this study the concordances between resistance to kanamycin, tobramycin, gentamicin and the presence of[ aac(6')-Ie- aph(2') and aph(3')-III] genes were 65%,and the concordances between[ aac(6')-Ie- aph(2') , aph(3')-III and ant(4')-I] genes and resistance to kanamycin, tobramycin, gentamicin were 57%. Conclusion:Our study shows that periodic hospital surveillance is essential in Iran. In particular, given the alarming rate of aminoglycoside resistance among the MRSA isolates, the surveillance of aminoglycoside resistance strains carrying the AME genes is of crucial importance in Tehran hospital.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :سیما سادات سیدجوادی
کلیدواژه ها (انگلیسی):Staphylococcus aureus ; AMEs ; MRSA ; ICU
Subjects:QW Microbiology and Immunology
QZ Pathology
WX Hospital and other health Facilities
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4582
Deposited By: MS Soghra Golmaghani
Deposited On:07 Jun 1392 09:33
Last Modified:07 Jun 1392 09:33

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