مقایسه روش دستی برای تشخیص آلودگی های گوناگون باکتریایی در فرآورده ها

دبیرمقدم, ابوالفضل and رازجو, فرهاد (1391) مقایسه روش دستی برای تشخیص آلودگی های گوناگون باکتریایی در فرآورده ها. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Comparison of the Bact/Alert Blood Culture System and Manual Culture Methods for Detection of Aerobic and Facultative Anaerobic Bacterial Contamination in Platelet Concentrates

English Abstract

Background & Objectives: Bacterial contamination evaluation of blood products is regarded as important point of blood safety. Since platelets should be stored at 20 to 24C° that makes them an excellent growth medium for bacteria, it is mentioned as a major problem in transfusion medicine. For reducing this risk, FDA has approved the Bact/Alert (Biomerieux- France) for screening the platelet units. This study attempt to compare the Bact /Alert system and manual culture methods regarding length of time in hours to detection. Methods: In this interventional and diagnostic study 15 platelet unit were selected randomly among 1332 unit and inoculated by 10 CFU/mlof various bacteria which contaminate platelet units routinely including streptococci, serratia marcescens, enterobacter cloaceae, corynebacterium diphteroied , staphylococci aureus and staphylococci epidermidis . Then these units with other platelet units were tested by Bact/Alert system and manual Methods as unknown sample. Results: Regarding the shortage of platelet unit expiration time if length of time in hours to detection is used as a basis for comparison , the Bact/Alert system is significantly superior to manual Methods , insofar as it detects positives significantly faster .The medium length of time in hours for detecting the aerobic bacterias by Bact/Alert system is 31 hours ( SD: ±8) following inoculation of samples to medium bottles in comparison 61 hours( SD: ±11) by manual Methods .This time is by Bact/Alert system is nearly 2 time faster than manual Methods. Conclusion: Bact/Alert culture system in compare with manual Methods is more rapid and accurate for detection of bacterial contamination and so platelet safety will improve by using this system .

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :ابوالفضل دبیرمقدم
کلیدواژه ها (انگلیسی):Platelet Concentrate; Bacterial Contamination; Bact/Alert; Manual Culture
Subjects:QW Microbiology and Immunology
WH Hemic and Lymphatic System
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4588
Deposited By: MS Soghra Golmaghani
Deposited On:07 Jun 1392 12:24
Last Modified:07 Jun 1392 12:24

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