هيدروليز اسيدي رقيق سرخس آبزي آزولا( Azolla Sp) براي توليد بيواتانل

شرفی, شهرام and آذین, مهرداد and ماشینجیان مرادی, علی and خنافری, آنیتا and فاطمی, سید محمدرضا (1391) هيدروليز اسيدي رقيق سرخس آبزي آزولا( Azolla Sp) براي توليد بيواتانل. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Dilute Acid Hydrolysis of Freshwater Fern (Azolla Sp.) For Production of Bioethanol

English Abstract

Background & Objectives: For controlling the invasive growth and reduction of unfavorable effects of aggressor freshwater plant (Azollasp.) in Anzali wetland and using it as a new feedstock biomass, dilute acid hydrolysis of it was performed for production of bioethanol. Methods: Samples were taken from different locations in Anzali wetland. After drying and milling, samples were digested by dilute acid and enzymatic methods. Dilute acid hydrolysis was done by autoclaving the ferns and also by a high pressure device (BatchSynth® Microwave synthesizer). Effect of different temperatures (in BatchSynth), times (in autoclave) and acid concentrations (in both) were compared. Cellulase treatment (Cellubrix®) was used for enzymatic hydrolysis of the residues. Reducing sugar, glucose and furfural contents were compared in hydrolysates. Results: Highest amount of glucose (4.4% w/w) and reducing sugars (17.73%) were produced after autoclaving the samples for 60 and 20 min, respectively, by addition of 1.67% sulfuric acid. In Microwave Batchsynth system, maximum glucose (5.1% w/w) and reducing sugar (13.26% w/w) were produced at 200 and 180○C, respectively. Maximum amount of furfural (1.54 g/L), as an inhibitor of cell growth, was produced in microwave Batchsynth, while its content was insignificant after autoclaving the samples. Conclusion: Statistical comparison showed significant (P≤0.05) difference in glucose production, which is important for fermentation. Total reducing sugars and glucose content were increased by optimizing acid concentration, time and temperature. By autoclaving, 1.67 % acid was best for total sugar and glucose production. By using Batchsynth microwave, increasing temperature led to increasing furfural, significantly (P≤0.05). Furfural strongly inhibits fermentation. Therefore, regarding energy and time consumption, inhibitor content and produced sugar, application of autoclave system for sugar production showed to be superior to high pressure microwave treatment of Azola.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :شهرام شرفی
کلیدواژه ها (انگلیسی):Temprature ; Acid Hydrolysis ; Azolla ; Biofuel
Subjects:WA Public Health > WA 30 Social, Economic, and Environmental factor in public health
WA Public Health > WA 30 Social, Economic, and Environmental factor in public health

QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4631
Deposited By: MS Soghra Golmaghani
Deposited On:21 Jun 1392 07:52
Last Modified:07 Jul 1393 06:57

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