تشخیص بروسلا آبورتوس و بروسلا ملی تنسیس در نمونه های خون انسان به روش Multiplex PCR

میرنژاد, رضا and پیرانفر, وهاب and احمدی, علی and گلمحمدی, رضا (1391) تشخیص بروسلا آبورتوس و بروسلا ملی تنسیس در نمونه های خون انسان به روش Multiplex PCR. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Simultaneous Detection of Brucella abortus and Brucella melitensis in Human Blood Samples by Multiplex PCR

English Abstract

Background & Objectives: There are several methods to detect Brucella spp. from clinical samples, but most of these methods do not have the power of rapid and simultaneous detection of Brucella species from clinical samples. This study was designed to evaluate rapid and simultaneous detection of Brucella abortus, Brucella melitensis in human blood samples through the Multiplex PCR Methods. Methods: Human blood samples were collected from 52 patients. Following DNA extraction, PCR assay were performed, using three primers that could simultaneously identify and differentiate two major species of pathogenic Brucella in humans and animals. These primer set, amplified a 494 bp fragment (B. abortus) and 733 bp fragment (B. melitensis). Finally, to confirm PCR products, In addition to the products sequence, RFLP was performed on PCR products using restriction enzymes. Results: of the 52 blood samples tested, 25 sample (48%) showed positive reactions in multiplex-PCR. Twelve samples were positive for B. abortus (48%) and 13 for B. melitensis (52%). Conclusion: In case where specific primers were utilized, multiplex-PCR has proved to be a simple, fast, and relatively inexpensive Methods for simultaneous detection of all three clinically important Brucella spp.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :رضا میرنژاد
کلیدواژه ها (انگلیسی):Brucella abortus ; Brucella melitensis ; Multiplex PCR
Subjects:QW Microbiology and Immunology
WH Hemic and Lymphatic System
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4660
Deposited By: MS Soghra Golmaghani
Deposited On:26 Jun 1392 09:05
Last Modified:26 Jun 1392 09:05

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