title

آنالیز ملکولی ویبریو کلرا واریانت های التور جدا شده از اپیدمی های بیماری وبا در سال 1384 در ایران به روش REP-PCR و ERIC-PCR

سهراب نژاد, علی and اکبری قمی, مصطفی and ملکی, عباس and غفوریان, سبحان and صادقی فرد, نورخدا (1391) آنالیز ملکولی ویبریو کلرا واریانت های التور جدا شده از اپیدمی های بیماری وبا در سال 1384 در ایران به روش REP-PCR و ERIC-PCR. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Molecular Analysis of Vibrio Cholerae El Torv Ariants Isolated from Outbreaks During 2005 in Iran by REP-PCR and ERIC-PCR

English Abstract

Background & Objectives: In the present study, we analyzed the PCR patterns of Vibrio cholerae El Tor variants isolated from outbreaks during 2005 in Iran by REP-PCR and ERIC-PCR. Methods: Thirty nine El Tor variants were obtained from outbreaks during 2005 in Iran. After detection of isolates by biochemical methods, and serotyping, chromosomal DNA was extracted by standard phenol/chloroform methods. REP-PCR was carried out using Rep 1R- I and Rep 2-I primers, also ERIC-PCR was done by using ERIC -1R and ERIC – 2. The PCR product was run and visualized in 1.5% agarose gels stained with ethidium bromide. Results: Thirty nine El Tor variants were analyzed and PCR Product sizes by REP-PCR methods were between 250-3500 bp and 5 different PCR patterns were detected. The most common pattern by REP-PCR was observed among 26 (66.7%) isolates. By ERIC-PCR 7 different patterns were detected. Conclusion: Results of this study showed that most of strains have similar pattern by REP-PCR and ERIC-PCR. It is assumed that these strains have the same origin.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :علی سهراب نژاد
کلیدواژه ها (انگلیسی):Vibrio cholerae ; Molecular Typing ; Iran
Subjects:QW Microbiology and Immunology
WI Digestive System
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4714
Deposited By: MS Soghra Golmaghani
Deposited On:08 Jul 1392 11:52
Last Modified:08 Nov 1397 08:21

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