مطالعه الگوی حساسیت آنتی بیوتیکی Acinetobacter baumannii تشکیل دهنده بیوفیلم به سیپروفلوکسازین

Title

Study of Antibacterial Susceptibility Pattern of Biofilm Producing Acinetobacter Baumannii Against Ciprofloxacin

English Abstract

Background & Objectives: Biofilms are highly structured communities of bacteria attached to a surface that recognized as a common cause of human infection. Acinetobacter baumannii biofilms has emerged worldwide as an important nosocomial infecting factor that is difficult to treat because of its multiple antibiotic resistance. Until 1988, quinolones had good activity against Acinetobacter strains. However, resistance to these antibiotics has developed rapidly in clinical isolates. This study presents the lowest concentration of ciprofloxacin that can inhibit the biofilm formation by Acinetobacter baumannii isolates. Methods: 75 isolates from burn wounds and catheters was evaluated for biofilm formation ability according to Srdjan Stepanović et al, (1999) in Tehran. MICs were determined by a broth microdilution methods which 100 µl of each bacterial were added to each well containing 100 µl ciprofloxacin with different dilutions. plates were incubated 24h at 37°C. Results: Results showed that 19% of isolates can form strong biofilms, 11% moderate biofilms, 48% week biofilms, and 22% are non-biofilm forming isolates. The lowest concentration of ciprofloxacin that inhibit biofilm formation for 69 isolates were higher than 4 µg/ml and for 5 isolates were lower than 1 µg/ml and for 1 isolate was 2 µg/ml. Conclusion: In conclusion, the results presented in this study demonstrated the high prevalence of biofilm formation by A. baumanii isolates, validate the importance of biofilms in nosocomial infections, and the potential of high concentrations of ciprofloxacin (128≥) to inhibit attachment and biofilm production by Acinetobacter baumannii isolates on abiotic surfaces.

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