title

نقش آب پنیر محتوی باکتریهای پروبیوتیکی لاکتوباسیلوس کازئی و بیفیدوباکتریوم بیفیدوم در درمان و پیشگیری از کولیت در مدل حیوانی

دیداری, تینا and سلکی, سارا and عبداللهی, محمد (1391) نقش آب پنیر محتوی باکتریهای پروبیوتیکی لاکتوباسیلوس کازئی و بیفیدوباکتریوم بیفیدوم در درمان و پیشگیری از کولیت در مدل حیوانی. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Role of Whey Containing Probiotics Bacteria of Lactobacillus casei and Bifidobacterium Bifidum in Treatment and Prevention of Colitis on Animal Model

English Abstract

Background & Objectives: Inflammatory bowel disease (IBD) is a severe inflammation in the gastrointestinal tract and consists of two forms: ulcerative colitis (UC) and Crohn’s disease (CD). Probiotics have anti-inflammatory properties for curing gastrointestinal disorders like inflammatory bowel disease (IBD). Gram positive lactic acid bacteria such as Lactobacilli strains and Bifidobacteria strains are primary microorganisms that classified as probiotics. Previous studies showed that Lactobacillus casei and Bifidobacterium bifidum reduced the levels of inflammatory biomarkers and modulate immune responses on murine models of colitis, separately. The aim of this study was to investigate the synergistic anti-inflammatory effects of whey cultured L. casei and B. bifidum on IBD management. Methods: In our study we cultured L.caseiand, B.bifidum in whey, separately and then combined these probiotics together and administered orally to male Wiastar rats for 10 days. Colitis was induced by rectal instillation of trinitrobenzene sulphonic acid (TNBS). Animals were divided into 5 groups included normal (non-colitis), negative control (without any treatment), positive control (dexamethasone, 1 mg/kg/day, orally), prevention (1×108 cfu L. casei and 1×108 cfu B. bifidum/day, 10 days before TNBS-induced colitis), treatment (1×108 cfu L. casei and 1×108 cfu B. bifidum/day, 10 days after TNBS-induced colitis). Biopsy specimens were obtained from distal colon and then inflammatory markers consisting of tumor necrosis factor-α (TNF-α), myeloperoxidase (MPO) and lipid peroxidation (LPO) were identified. Results: In treatment group, macroscopic scores reduced significantly and levels of TNF-α, MPO and LPO decreased but prevention group did not show positive effect to ameliorate colonic lesions. Conclusion: These data indicated that combination of L. casei and B. bifidum could alleviate colon damage and inflammatory markers in treatment colitis. Additional studies required to define the role of another probiotics combination to improve IBD.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :تینا دیداری
کلیدواژه ها (انگلیسی):Whey ; Lactobacillus casei ; Bifidobacterium Bifidum ; Colitis
Subjects:QW Microbiology and Immunology
WI Digestive System
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4820
Deposited By: MS Soghra Golmaghani
Deposited On:01 Aug 1392 12:13
Last Modified:01 Aug 1392 12:49

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