شناسایی عفونت کمپیلوباکتر در بیماران اسهالی به روش PCR

Title

Identification of Campylobacter Infection in The Patients with Diarrhea by PCR

English Abstract

Background & Objectives: Campylobacter is a gram-negative, spiral and microaerophilic bacteria that it is a common diarrhea in humans cause of acute disease. The aim of this study was to identification of campylobacter infection in the patients with diarrhea by PCR. Methods: Stool samples from 117 patients with diarrhea were collected and dissolved in 500µl PBS and 100µl added to thioglycollate medium was incubated over night under microaerophilic environment at 42 oC. Then 100µl of samples were cultured in blood free campylobacter agar base medium and were incubated at 42oC under microaerophilic conditions for 48h. C.jejuni and C.coli colonies were tested for biochemical characters, Gram staining, oxidase, cataalase and hippurate hydrolysis. DNA was extracted from pure bacterial cultures. The hipO and asp genes were used for identification of C.jejuni and C.coli respectively. Genes amplified by PCR. Results: C.jejuni was isolated from stool samples of 9 patients (7.69% ) by PCR and C.coli was isolated from stool samples of 4 patients by PCR (3.41% ). Conclusion: The findings suggest that PCR is a rapid diagnostic tool for detecting C.jejuni and C.coli infection in diarrhea patients.

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