بررسی میزان فراوانی ژن های مقاومت نسبت به آمینوگلیکوزید و بتالاکتام های وسیع الطیف در سودوموناس آئروژینوزا جدا شده از بخش های مختلف بیمارستان

Title

Frequency of the Resistance Genes Against Aminoglycoside and Extended Spectrum Beta Lactamase (ESBL) in the Pseudomonas aeroginosa

English Abstract

Background & Objectives: Pseudomonas aeroginosa is common hospitalized pathogens. Resistance of this bacterium against different antibiotics which have been reported from the various regions. The objective of this study is to determine the antibiotic susceptibility of the Pseudomonas aeroginosa strains isolated from the various wards of the hospital and the presence of the blaoxa-50, blaoxa-2, pstS, aadA, aac(6') , aadE and aph(2'') genes. Methods: In this study, 35 strains of Pseudomonas aeroginosa were collected from different wards of the Shahid Rejaei hospital of Tonekabon within 2011-2012. In order to determine the resistance of strains, antibiogram test was carried out by the Methods of the disk diffusion. In order to the presence of the studied genes, the Specific primers were used and the PCR technique was applied to amplification the above genes. Results: The results obtainedforantibiotics susceptibility of Pseudomonas aeroginosa isolated from hospital samples by disk diffusion methods indicated that all strains were resistant to Cophalexine, Cephalotine, Ampiciline, Penicilin, Amoxicilin and Co-amoxycelav. Also, they showed different levels of resistant to other antibiotics including 85.7% to Neomycine, 42.8% to Toberamycin, 28.5% to Cephteriaxon, 20% to Cephtazidium and 2.8% to Imipenem. All Pseudomonas aeroginosa isolates were sensitive to Ciprofloxacin, Gentamicin and Amicacin. Of total 35 studied strains, Psts, bloa oxa 50 and addE genes was observed in 35 (100%), 34 (97.1%) and 33 (94.2%) strains, respectively. Also, the aac(6'), aph(2"), aaA and bla oxa2 genes were not identified in the none of the isolated starins. Conclusion: With regard to the high percentage of the resistance of isolated Pseudomonas aeroginosa to the antibiotics of the group of the Aminoglycoside and extended spectrum beta lactamase, the accurate performance of the anti-biogram tests before the prescription of the antibiotic in the treatment of the infections resulted from these bacteria is an unavoidable necessity.

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