بررسي مقاومت آنتي بيوتيكي در اشريشياكلي جدا شده از عفونت ادراري در بيماران سرپايي در تهران

بابایی کسمایی, زهرا ، آرش كيا, آرش ، فروهش تهرانی, هما ، امیر مظفری, نور (1391) بررسي مقاومت آنتي بيوتيكي در اشريشياكلي جدا شده از عفونت ادراري در بيماران سرپايي در تهران. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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آدرس اینترنتی رسمی : http://congress.arums.ac.ir/index.php/IICM/5/sched...

عنوان انگليسي

Frequency of Broad-Spectrum Beta-Lactamase Genes Among Escherichia coli Strains Isolated from Urinary Tract- Infected Outpatients in Tehran

خلاصه انگلیسی

Background & Objectives: Escherichia coli is the most prevalent agent of urinary tract infection in both outpatients and hospitalized patients. In recent years, due to extended-spectrum β-lactamases (ESBLs), cases of resistance to antimicrobial agents especially to cephalosporin has raised considerably. This study was aimed to determine the survey of CTX-M، SHV، TEM، OXA-1, PER-2, VEB-1 in E. coli isolated from outpatients with UTI at selected laboratories in Tehran, Iran. Methods: This study that we had done during 6 months, from the first of October to the end of March. After isolation test, we identified 123 samples (13.63%) from among of 1677 urine samples as E. coli and then by using double disk Methods, 56 samples (45.52%) have recognized as ESBL and we done PCR methods on this samples in order to examine studying genes after gene extraction with special kit primarily by using universal PCR and for being sure of accurate gene extraction. Results: Among 1677 urine samples, 13.63% are E. coli and after double disk methods, 45.52% are positive according to ESBL Molecular PCR also shows 51.8% CTX-M, 7.1% TEM, 3.6% SHV, 42% OXA-1, 8.9% PER-2 but we didn't find VEB-1. Conclusion: Because of susceptibility of genes, the curing of infection causing by this bacteria fails despite of the existence of convenient recognition ways. According to statistical view, there is a direct relation between gender and age in this illness, so we can say that there is a direct relation between the ability in ESBL production and the existence of examining genes.

نوع سند :موضوع کنفرانس یا کارگاه (پوستر )
زبان سند : انگلیسی
نویسنده مسئول :زهرا بابایی کسمایی
کلید واژه ها (انگلیسی):ESBL ; E. coli, CTX-M; OXA-1 ; Doubble Disk ; Universal PCR
موضوعات :QW میکروب شناسی و ایمنی شناسی
WJ سیستم ادراری
بخش های دانشگاهی :معاونت تحقیقات و فناوری > مديريت تحقیقات و فناوری و اطلاع رساني > مدیریت همایش ها و کنگره های دانشگاه
کد شناسایی :4926
ارائه شده توسط : خانم صغری گلمغانی
ارائه شده در تاریخ :28 آبان 1392 14:18
آخرین تغییر :31 خرداد 1393 08:33

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