title

فراوانی ژن های اکسفولیاتیوتوکسین A و B در استافیلوکوکوس های جدا شده از نمونه های بالینی

زارعی کوشا, روح اله and ایمانی فولادی, عباسعلی and رنجبر, رضا (1391) فراوانی ژن های اکسفولیاتیوتوکسین A و B در استافیلوکوکوس های جدا شده از نمونه های بالینی. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Distribution of Exfolaitive Toxin A and B Genes in Staphylococcus aureus Isolated from Clinical Specimens

English Abstract

Background & Objectives: Some of Staphylococcus aureus strains produced difference type of exfoliative toxins. Exfoliative toxins (also known as “epidermolytic” toxins) are particularly interesting virulence factors of S. aureus. Production of exfoliative toxin by these strains of S. aureus can be determined using a range of currently available diagnostic tests, including PCR. These tests were carried out in researching laboratories. In this study we are detecting ETs A&B in Staphylococcus aureus isolated from clinical speicmens Baqiyatallah Medical University of Tehran.The principal aim of this study is evaluation abundance of ETs A & B. Methods: 200 strains of S. aureus isolated from clinical samples were subjected to PCR. The strains were maintained on tryptic soy agar plates and according to the methods of genomic DNA extraction kit (bioneer Co. Korea) DNA extraction was performed. We were designed ETA and ETB primers and evaluated by blast software. Results: After detection of eta and etb genes by PCR methods, the products were analyzed on agerose gel electrophoresis. According to SPSS software and statistics table, ETA -producing strains have more prevalent than those expressing ETB. Conclusion: Our results have revealed that eta genes were more prevalent than the etb genes among S. aureus isolates. It’s seems then probably presence of eta&etb genes have significant relative with clinical samples. Detection Ets have important uses, on their molecular mechanisms and epidemiology and true nature of the superantigenic properties of Ets and their roles and relationship to their pathogenesis remain.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :روح اله زارعی کوشا
کلیدواژه ها (انگلیسی):Exfoliative ToxinA&B ; S.aureus ; PCR
Subjects:QW Microbiology and Immunology
WX Hospital and other health Facilities
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4934
Deposited By: MS Soghra Golmaghani
Deposited On:29 Aug 1392 10:08
Last Modified:29 Aug 1392 10:08

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