title

تشخیص سریع حساسیت به اتامبوتول در سویه های مایکوباکتریوم توبرکلوزیس ایزوله شده از بیماران مسلول با استفاده از روش PCR-RFLP

طاهر احمدی, معصومه and ارجمندزادگان, محمد and نظری, راضیه and شجاع پور, مانا and احمدی, اعظم and مودب, سید رضا (1391) تشخیص سریع حساسیت به اتامبوتول در سویه های مایکوباکتریوم توبرکلوزیس ایزوله شده از بیماران مسلول با استفاده از روش PCR-RFLP. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Rapid Detection of Susceptibility to Ethambutol in Clinical Mycobacterium Tuberculosis Isolated from Tuberculosis Patients by PCR- RFLP

English Abstract

Background & Objectives: Ethambutol is a key medicine to treat tuberculosis, and resisting against it has been increasingly reported. In this paper PCR-RFLP methods to quickly identify the resistance of Mycobacterium tuberculoses stains against the ethambutol has been investigated. Methods: In this study, 127 stains in Arak Research Center, 60 stains were investigated; then DNA was separated by Chelex100 methods. PCR was executed for amplification of 167 bp of embB gene with the help of exclusive primers. PCR product, using HaeIII, was RELPed and the bands patter was determined. The section was sequenced in a few samples, and it was compared to the presented methods as golden standard. Results: Out of 60 studied stains, 43 were phenotypically resistant to ethambutol, and 17 were sensitive. PCR revealed the band 167, which showed the correct selection of primers and the appropriate plan of amplification. Out of 43 resistant stains, 19 stains were diagnosed to have mutation in ATG-Met codon 360 using RFLP Methods, and 24 were found to be non-mutant. Conclusion: Results show that PCR-RFLP can be a simple and quick methods to diagnose the ethambutol -sensitivity in Mycobacterium tuberculosis stains.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :معصومه طاهر احمدی
کلیدواژه ها (انگلیسی):Ethambutol ; Mycobacterium tuberculosis ; PCR ; RFLP
Subjects:QW Microbiology and Immunology
WC Communicable Diseases
WF Respiratory System
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4936
Deposited By: MS Soghra Golmaghani
Deposited On:29 Aug 1392 10:22
Last Modified:29 Aug 1392 10:22

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