تاثیر شرایط کشت یک سویه جدید تیوباسیلوس در اکسیداسیون آهن

رخشانی, یاسین and راهب, جمشید and تابنده, فاطمه and سبکبار, آذر and عربنژاد, محمود (1391) تاثیر شرایط کشت یک سویه جدید تیوباسیلوس در اکسیداسیون آهن. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Effects of Culture Conditions of Fe-oxidation in a Newly-isolated Thiobacilus ferrooxidans

English Abstract

Background & Objectives: Bioleaching is a process utilizing microorganisms to extract heavy metals from ores. This process can be carried out by several groups of bacteria but the most commonly-used one is Acidothiobacillus. The main aimof this research was to isolate the local bacteria from Meydook ore in order to achieve the best strain. The effect of culture medium, temperature and pH on bioleaching process was also investigated. Methods: The mineral 9-K medium was utilized for bacterial growth and leaching. The samples of ore were treated with 0.1 N sulfuric acid then, 70 ml of resulting liquid was added to 200 ml of K-9 medium and incubated at 30° C. DNA extraction and PCR DNA was extracted using DNA extraction kit. The spacer region between 16s and 23s genes was amplified by PCR using forward and reverse primers of 27F and 1492R. Optimization of the medium Temperature, pH and culture medium components were optimized by statistical Methods and analyzed by Design-expert-6.0.10 software. Results: The indigenous bacterium Acidothiobacillus ferrooxidans was registered in NBCI gene bank with FR772290 number. Furthermore, the optimum culture conditions were obtained as follows: (NH4)2SO4 (1.5 g/L), KH2PO4 (0.34 g/L), MgSO4 (0.34 g/L), KCl (0.2 g/L), Ca(NO3)2 (0.01 g/L), FeSO4 7H2o (25g/L), pH (2.5), temperature (35 °C). Conclusion: It was demonstrated that the most effective factors on Fe-oxidation process by the bacterium were temperature , pH and the amount of FeSO4 7H2o.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :یاسین رخشانی
کلیدواژه ها (انگلیسی):Bioleaching ; Thiobacillus ferrooxidans ; Copper ; Optimization
Subjects:QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5028
Deposited By: MS Soghra Golmaghani
Deposited On:24 Sep 1392 09:30
Last Modified:24 Sep 1392 09:30

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