تشخیص مولکولی ژن های متالوبتالاکتاماز در سویه های پسودوموناس آیروژینوزای جدا شده از بیماران بستری در بیمارستان های اراک

Title

Molecular Detection of Metallo-β-Lactamase Genes in Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients in Arak

English Abstract

Background & Objectives: In 1966 Metallo-β-lactamase (MBL) was discoverd in Bacillus cereus for first time (1). MBL genes are located on chromosome and plasmids. These plasmids are placed on transposons or integrons. Aquired MBLs exist Enterobacteriace, Pseudomonas aeruginosa and Acintobacter baumanii. MBL genes can be transmitted horizontally, these genes cause carbapenem resistance in Enterobacteriace and other gram negative bacteria. Presence of VIM (Veronese Imipenemase), IMP (Imipenemase), SPM (Sao Paulo Metallo-β-lactamase), GIM (Germany Imipenemase) in P. aeruginosa cause resistance to imipenem, meropenem, anti-pseudomonal cephalosporins and anti-pseudomonal penicillins. The aim of this study was to determine the prevalence of MBL genes in P. aeruginosa strains isolated from hospitalized patients in Arak. Methods: 40 imipenem-resistant P. aeruginosa strains were collected from samples of different hospitalized patients in Arak hospitals. Molecular studies was done by Duplex-PCR to detect two MBL genes including blaVIM-1and blaIMP-1 in 40 imipenem-resistant P. aeruginosa strains. Results: blaVIM-1 and blaIMP-1 were detected in imipenem-resistant P. aeruginosa strains respectively 50 percent and 6.6 percent. Conclusion: According to this study frequency of blaVIM-1 in Arak is almost like what previous studies in other cities showed. But this seems to be first report of detection of blaIMP-1 in P. aeruginosa in Iran. Therefore it is necessary to control MBL-producing strains especially in hospitals.

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